Technologies of Agrobacterium plant transformation In planta

被引:14
作者
Chumakov, M. I. [1 ,2 ]
Moiseeva, E. M. [1 ]
机构
[1] Russian Acad Sci, Inst Biochem & Physiol Plants & Microorganisms, Saratov 410049, Russia
[2] Saratov NG Chernyshevskii State Univ, Saratov 410012, Russia
基金
俄罗斯基础研究基金会;
关键词
genetic transformation in planta; transgenic plants; Agrobacterium tumefaciens; MEDIATED GENE-TRANSFER; ARABIDOPSIS-THALIANA; FLORAL-DIP; VACUUM INFILTRATION; T-DNA; GERMINATING-SEEDS; L; WHEAT; POLLEN; TUMEFACIENS;
D O I
10.1134/S0003683812080017
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In this review, methods of Agrobacterium T-DNA transfer into plant cells in planta are discussed. The main focus is on the technologies of gene transfer into generative plant cells as a part of Agrobacterium T-DNA. The influence of the plant genotype, bacterial strain, vector construction type, inoculation medium composition, and the conditions of plant treatment with Agrobacterium on the efficiency of Agrobacterium transformation in planta is analysed. Based on literature and personal experimental data, the possible mechanism of Agrobacterium transformation of generative plant cells in planta is discussed.
引用
收藏
页码:657 / 666
页数:10
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