Processing of Structurally Heterogeneous Cryo-EM Data in RELION

被引：389
|
作者：
Scheres, S. H. W. [1 ]
机构：
[1] MRC Lab Mol Biol, Francis Crick Ave,Cambridge Biomed Campus, Cambridge, England
来源：
RESOLUTION REVOLUTION: RECENT ADVANCES IN CRYOEM | 2016年 / 579卷
基金：
英国医学研究理事会;
关键词：
PARTICLE ELECTRON CRYOMICROSCOPY; LIKELIHOOD-BASED CLASSIFICATION; BEAM-INDUCED MOTION; U4/U6.U5; TRI-SNRNP; 3-DIMENSIONAL RECONSTRUCTION; MAXIMUM-LIKELIHOOD; UCSF CHIMERA; MICROSCOPY; RESOLUTION; IMAGES;
D O I：
10.1016/bs.mie.2016.04.012
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This chapter describes algorithmic advances in the RELION software, and how these are used in high-resolution cryo-electron microscopy (cryo-EM) structure determination. Since the presence of projections of different three-dimensional structures in the dataset probably represents the biggest challenge in cryo-EM data processing, special emphasis is placed on how to deal with structurally heterogeneous datasets. As such, this chapter aims to be of practical help to those who wish to use RELION in their cryo-EM structure determination efforts.
引用
收藏
页码：125 / 157
页数：33
相关论文
共 50 条
  • [1] A microtubule RELION-based pipeline for cryo-EM image processing
    Cook, Alexander D.
    Manka, Szymon W.
    Wang, Su
    Moores, Carolyn A.
    Atherton, Joseph
    JOURNAL OF STRUCTURAL BIOLOGY, 2020, 209 (01)
  • [2] Higher Resolution with the Same Data - Tuning cryo-EM Refinement in Relion
    Forsberg, Bjorn O.
    Kimanius, Dari
    Lindahl, Erik
    BIOPHYSICAL JOURNAL, 2016, 110 (03) : 157A - 157A
  • [3] Processing of Cryo-EM Movie Data
    Ripstein, Z. A.
    Rubinstein, J. L.
    RESOLUTION REVOLUTION: RECENT ADVANCES IN CRYOEM, 2016, 579 : 103 - 124
  • [4] Accelerating the cryo-EM structure determination in RELION on GPU cluster
    YOU Xin
    YANG Hailong
    LUAN Zhongzhi
    QIAN Depei
    Frontiers of Computer Science, 2022, 16 (03)
  • [5] RELION: Implementation of a Bayesian approach to cryo-EM structure determination
    Scheres, Sjors H. W.
    JOURNAL OF STRUCTURAL BIOLOGY, 2012, 180 (03) : 519 - 530
  • [6] Accelerating the cryo-EM structure determination in RELION on GPU cluster
    Xin You
    Hailong Yang
    Zhongzhi Luan
    Depei Qian
    Frontiers of Computer Science, 2022, 16
  • [7] Accelerating cryo-EM Reconstruction of RELION on the New Sunway Supercomputer
    Xu, Jingle
    Fu, Jiayu
    Gan, Lin
    Chen, Yaojian
    Huang, Zhenchun
    Yang, Guangwen
    2022 IEEE INTL CONF ON PARALLEL & DISTRIBUTED PROCESSING WITH APPLICATIONS, BIG DATA & CLOUD COMPUTING, SUSTAINABLE COMPUTING & COMMUNICATIONS, SOCIAL COMPUTING & NETWORKING, ISPA/BDCLOUD/SOCIALCOM/SUSTAINCOM, 2022, : 129 - 138
  • [8] Accelerating the cryo-EM structure determination in RELION on GPU cluster
    You, Xin
    Yang, Hailong
    Luan, Zhongzhi
    Qian, Depei
    FRONTIERS OF COMPUTER SCIENCE, 2022, 16 (03)
  • [9] A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
    DiIorio, Megan C.
    Kulczyk, Arkadiusz W.
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2022, (179):
  • [10] Semi-automated selection of cryo-EM particles in RELION-1.3
    Scheres, Sjors H. W.
    JOURNAL OF STRUCTURAL BIOLOGY, 2015, 189 (02) : 114 - 122
12345