Bulked segregant analysis for relative water content to detect quantitative trait loci in wheat under drought stress

被引：6

作者：

Rad, M. R. Naroui ^{[1
,2
]}

Kadir, M. Abdul ^{[1
]}

Rafii, M. Y. ^{[3
]}

Jaafar, H. Z. E. ^{[4
]}

Naghavi, M. R. ^{[5
]}

机构：

[1] Univ Putra Malaysia, Agrotechnol Dept, Fac Agr, Serdang, Selangor, Malaysia

[2] Agr & Nat Resources Res Ctr, Sitan, Iran

[3] Univ Putra Malaysia, Inst Trop Agr, Serdang, Selangor, Malaysia

[4] Univ Putra Malaysia, Dept Crop Sci, Fac Agr, Serdang, Selangor, Malaysia

[5] Univ Tehran, Dept Agron & Plant Breeding, Fac Agr, Karaj, Iran

来源：

GENETICS AND MOLECULAR RESEARCH | 2012年 / 11卷 / 04期

关键词：

Drought tolerance; Bulked segregant analysis; QTL; SSR markers; Wheat; OSMOTIC ADJUSTMENT; WINTER-WHEAT; RESISTANCE; TOLERANCE; GENOTYPES; MARKERS; RICE;

D O I：

10.4238/2012.November.12.5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study was carried out to evaluate the genetic effect of quantitative trait loci (QTLs) conferring drought tolerance in wheat. A population of 120 F-2 individuals from the cross between the drought-tolerant S-78-11 and drought-sensitive Tajan cultivars were analyzed for their segregation under drought stress conditions. The relative water content under drought stress conditions exhibited continuous variation, indicating the minor gene effects on the trait. Single-marker analysis (SMA) was carried out to detect the main QTL association with drought tolerance. The SMA results revealed that the simple sequence repeat markers GWM182 and GWM292 on chromosome 5D and GWM410 on chromosome 5A exhibited significant association with drought tolerance, accounting for 30, 22, and 21% of the total variation, respectively. The 3 genetic loci, especially GWM182, can be used in marker-assisted selection methods in drought tolerance breeding in wheat.

引用

页码：3882 / 3888

页数：7

共 18 条

[1] A RE-EXAMINATION OF RELATIVE TURGIDITY TECHNIQUE FOR ESTIMATING WATER DEFICITS IN LEAVES [J].

BARRS, HD ;

WEATHERLEY, PE .

AUSTRALIAN JOURNAL OF BIOLOGICAL SCIENCES, 1962, 15 (03) :413-&

[2] OSMOTIC ADJUSTMENT AND GROWTH OF BARLEY GENOTYPES UNDER DROUGHT STRESS [J].

BLUM, A .

CROP SCIENCE, 1989, 29 (01) :230-233

[3] ISOTOPIC COMPOSITION OF PLANT CARBON CORRELATES WITH WATER-USE EFFICIENCY OF WHEAT GENOTYPES [J].

FARQUHAR, GD ;

RICHARDS, RA .

AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY, 1984, 11 (06) :539-552

[4] Bulked segregant analysis to detect main effect QTL associated with grain quality parameters in Basmati 370/ASD 16 cross in rice (Oryza sativa L) using SSR markers [J].

Govindaraj, P ;

Arumugachamy, S ;

Maheswaran, M .

EUPHYTICA, 2005, 144 (1-2) :61-68

[5]

Liu BH, 1998, STAT GENOMICS, P387

[6] Potential importance of water status traits for durum wheat improvement under Mediterranean conditions [J].

Merah, O .

JOURNAL OF AGRICULTURAL SCIENCE, 2001, 137 :139-145

[7] IDENTIFICATION OF MARKERS LINKED TO DISEASE-RESISTANCE GENES BY BULKED SEGREGANT ANALYSIS - A RAPID METHOD TO DETECT MARKERS IN SPECIFIC GENOMIC REGIONS BY USING SEGREGATING POPULATIONS [J].

MICHELMORE, RW ;

PARAN, I ;

KESSELI, RV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (21) :9828-9832

[8] COMPARISON OF PHYSIOLOGICAL METHODS TO ASSESS DROUGHT TOLERANCE IN OATS [J].

PELTONENSAINIO, P ;

MAKELA, P .

ACTA AGRICULTURAE SCANDINAVICA SECTION B-SOIL AND PLANT SCIENCE, 1995, 45 (01) :32-38

[9] Mapping QTLs associated with drought resistance in rice: Progress, problems and prospects [J].

Price, A ;

Courtois, B .

PLANT GROWTH REGULATION, 1999, 29 (1-2) :123-133

[10]

Röder MS, 1998, GENETICS, V149, P2007

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