Decreased expression of LncRNA SLC25A25-AS1 promotes proliferation, chemoresistance, and EMT in colorectal cancer cells

被引:56
作者
Li, Yuan [1 ,2 ]
Huang, Shengkai [1 ,2 ]
Li, Yan [1 ,2 ]
Zhang, Weilong [1 ,2 ]
He, Kun [3 ]
Zhao, Mei [1 ,2 ]
Lin, Hong [1 ,2 ]
Li, Dongdong [1 ,2 ]
Zhang, Honggang [4 ,5 ]
Zheng, Zhaoxu [5 ,6 ]
Huang, Changzhi [1 ,2 ]
机构
[1] Peking Union Med Coll, Dept Etiol & Carcinogenesis, Canc Inst & Hosp, Beijing 100021, Peoples R China
[2] Peking Union Med Coll, State Key Lab Mol Oncol, Canc Inst & Hosp, Beijing 100021, Peoples R China
[3] Peking Union Med Coll Hosp, Dept Gastroenterol, Beijing 100730, Peoples R China
[4] Chinese Acad Med Sci, Canc Inst & Hosp, Dept Med Oncol, Beijing 100021, Peoples R China
[5] Peking Union Med Coll, Beijing 100021, Peoples R China
[6] Chinese Acad Med Sci, Canc Inst & Hosp, Dept Abdomen Surg, Beijing 100021, Peoples R China
基金
中国国家自然科学基金;
关键词
Colorectal cancer; Long non-coding RNA; SLC25A25-AS1; Chemoresistance; EMT; LONG NONCODING RNA; HEPATOCELLULAR-CARCINOMA; IN-VIVO; ACTIVATION; METASTASIS; KINASE; ERK; IDENTIFICATION; TRANSITIONS; ACQUISITION;
D O I
10.1007/s13277-016-5254-0
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Increasing evidence suggests that long non-coding RNAs (lncRNAs) are aberrantly expressed in colorectal cancer (CRC); however, only few CRC-related lncRNAs have been characterized. In this study, we aimed to dig out potential dysregulated lncRNAs that are highly involved in CRC development. Using a lncRNA-mining approach, we performed lncRNA expression profiling in a large CRC cohort from Gene Expression Ominus (GEO), GSE39582 test series (N = 585). We identified 31 downregulated lncRNAs and 16 upregulated lncRNAs from the GSE39582 test series patients (566 tumor patients and 19 normal controls). The reliability of lncRNA expression profiles was further confirmed by RT-qPCR in carcinoma tissues and paired adjacent normal tissues from 30 CRC patients, also in the serum from 109 CRC patients, and 99 normal individuals. We demonstrated that the expression of SLC25A25-AS1, which has not been reported previously, was significantly decreased in both the tumor tissues (27 out of 30) and serum of CRC patients. SLC25A25-AS1 overexpression significantly inhibited proliferation and colony formation in colorectal cancer cell lines, and downregulation of SLC25A25-AS1 obviously enhanced chemoresistance and promoted EMT process in vitro associated with Erk and p38 signaling pathway activation. Therefore, SLC25A25-AS1 was determined to play a tumor suppressive role in CRC. Our results might provide a lncRNA-based target for CRC treatment.
引用
收藏
页码:14205 / 14215
页数:11
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