Development and characterization of new microsatellites for walnut (Juglans regia)

被引：19
作者：
Zhang, Z. Y. [1 ]
Han, J. W. [1 ]
Jin, Q. [1 ]
Wang, Y. [1 ]
Pang, X. M. [1 ]
Li, Y. Y. [1 ]
机构：
[1] Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Lab Tree Breeding, Natl Dev & Reform Commiss, Beijing, Peoples R China
来源：
GENETICS AND MOLECULAR RESEARCH | 2013年 / 12卷 / 04期
关键词：
EST-SSR; Juglans regia; Juglans nigra; Carya cathayensis; Carya dabieshanensis; Annamocarya sinensis; SIMPLE SEQUENCE REPEATS; EST-SSR MARKERS; GENETIC DIVERSITY; DNA; IDENTIFICATION; DATABASES;
D O I：
10.4238/2013.October.18.10
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The expressed sequence tag (EST) database represents a potentially valuable resource for the development of simple sequence repeat (SSR) markers for use in evolutionary studies. EST-SSRs reveal polymorphisms not only within the source taxon, but in related taxa as well. In this paper, we describe a case study in which the publicly available walnut (Juglans regia) EST database was used to develop SSR markers for use in the genetic analysis of the widespread Juglans nigra and Carya cathayensis and an endangered species Annamocarya sinensis. A total of 7262 unigenes, including 1911 contigs and 5351 singletons, were obtained from 13,559 ESTs retrieved from the NCBI database. The 7262 unigenes were further reduced to 706 EST-SSR sequences containing 805 SSR loci. Then, 309 EST-SSR primers were randomly designed, and 77 were identified with five high across-species transferability cross-species: namely, J. regia, J. nigra, C. cathayensis, Carya dabieshanensis, and A. sinensis. Thirteen highly polymorphic EST-SSRs were further used for genetic analyses in these above five species.
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收藏
页码：4723 / 4734
页数：12
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