Development and validation of a new UPLC-PDA method to quantify linezolid in plasma and in dried plasma spots

被引:31
作者
Baietto, Lorena [1 ]
D'Avolio, Antonio [1 ]
Ariaudo, Alessandra [1 ]
Corcione, Silvia [1 ]
Simiele, Marco [1 ]
Cusato, Jessica [1 ]
Urbino, Rosario [1 ]
Di Perri, Giovanni [1 ]
Ranieri, V. Marco [2 ,3 ]
De Rosa, Francesco Giuseppe [1 ]
机构
[1] Univ Turin, Amedeo di Savoia Hosp, Infect Dis Unit, Dept Med Sci, I-10149 Turin, Italy
[2] Univ Turin, San Giovanni Battista Hosp, Dept Anesthesia, I-10126 Turin, Italy
[3] Univ Turin, San Giovanni Battista Hosp, Dept Intens Care Med, I-10126 Turin, Italy
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2013年 / 936卷
关键词
Linezolid; Dried plasma spots; HPLC; UPLC; Therapeutic drug monitoring TDM; Human plasma; Stability; PERFORMANCE LIQUID-CHROMATOGRAPHY; HIV-INFECTED PATIENTS; BLOOD SPOT; SIMULTANEOUS QUANTIFICATION; RESISTANT TUBERCULOSIS; ANTIRETROVIRAL AGENTS; ULTRAVIOLET DETECTION; MS/MS METHOD; LC-MS/MS; HPLC-MS;
D O I
10.1016/j.jchromb.2013.08.003
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Linezolid is an oxazolidinone antibiotic used for the treatment of pneumonia and uncomplicated and complicated skin and soft tissues infections caused by Gram positive bacteria. It is also used as second line agent in multi-drug resistant tuberculosis. Therapeutic drug monitoring (TOM) of linezolid represents a valid tool in clinical practice to optimize therapy, especially in critically ill patients. Spreading of TDM is mainly limited by high costs shipment and lack of laboratories that offer a TDM service. To overcome these problems, the use of dried plasma spots or dried blood spots is increasing. The aim of this work was to develop and validate a new chromatographic method to analyze linezolid in plasma and in dried plasma spots and to evaluate the correlation between the two extraction methods. Linezolid extraction from plasma and from dried plasma spots was obtained using acetonitrile. Quinoxaline was used as internal standard. Analysis was performed by an ultra performance liquid chromatography (UPLC) system coupled with photo diode array (PDA) detector, at 254 nm. Both analytical methods were linear (r(2)>0.999) over the calibration range of 30-0.117 mg/L. Limit of quantification and limit of detection were 0.117 mg/L and 0.058 mg/L, respectively. Intra and inter-day precision (R.S.D.%) and accuracy (%) were <15%. Long term stability of linezolid in dried plasma spots showed absence of degradation at room temperature (20-25 degrees C) and at 4 degrees C, for at least one month. Linear regression analysis confirmed that the two methods of extraction have good correlation. Thus they are suited for TDM of linezolid and for pharmacokinetic studies. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:42 / 47
页数:6
相关论文
共 34 条
[1]   Development and validation of a new method to simultaneously quantify triazoles in plasma spotted on dry sample spot devices and analysed by HPLC-MS [J].
Baietto, Lorena ;
D'Avolio, Antonio ;
Marra, Cristina ;
Simiele, Marco ;
Cusato, Jessica ;
Pace, Simone ;
Ariaudo, Alessandra ;
De Rosa, Francesco Giuseppe ;
Di Perri, Giovanni .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2012, 67 (11) :2645-2649
[2]   Development, Validation, and Routine Application of a High-Performance Liquid Chromatography Method Coupled with a Single Mass Detector for Quantification of Itraconazole, Voriconazole, and Posaconazole in Human Plasma [J].
Baietto, Lorena ;
D'Avolio, Antonio ;
Ventimiglia, Giusi ;
De Rosa, Francesco Giuseppe ;
Siccardi, Marco ;
Simiele, Marco ;
Sciandra, Mauro ;
Di Perri, Giovanni .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2010, 54 (08) :3408-3413
[3]   Development and validation of a simultaneous extraction procedure for HPLC-MS quantification of daptomycin, amikacin, gentamicin, and rifampicin in human plasma [J].
Baietto, Lorena ;
D'Avolio, Antonio ;
De Rosa, Francesco Giuseppe ;
Garazzino, Silvia ;
Michelazzo, Marianna ;
Ventimiglia, Giusi ;
Siccardi, Marco ;
Simiele, Marco ;
Sciandra, Mauro ;
Di Perri, Giovanni .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 396 (02) :791-798
[4]   Simultaneous Quantification of Linezolid, Rifampicin, Levofloxacin, and Moxifloxacin in Human Plasma Using High-Performance Liquid Chromatography With UV [J].
Baietto, Lorena ;
D'Avolio, Antonio ;
De Rosa, Francesco Giuseppe ;
Garazzino, Silvia ;
Patanella, Samantha ;
Siccardi, Marco ;
Sciandra, Matiro ;
Di Perri, Giovanni .
THERAPEUTIC DRUG MONITORING, 2009, 31 (01) :104-109
[5]   High-performance liquid chromatographic method for simple and rapid determination of linezolid in human plasma [J].
Boak, Lauren M. ;
Li, Jian ;
Nation, Roger L. ;
Rayner, Craig R. .
BIOMEDICAL CHROMATOGRAPHY, 2006, 20 (08) :782-786
[6]   Linezolid (ZYVOX), the first member of a completely new class of antibacterial agents for treatment of serious gram-positive infections [J].
Brickner, Steven J. ;
Barbachyn, Michael R. ;
Hutchinson, Douglas K. ;
Manninen, Peter R. .
JOURNAL OF MEDICINAL CHEMISTRY, 2008, 51 (07) :1981-1990
[7]   Pharmacokinetic-pharmacodynamic evaluation of daptomycin, tigecycline, and linezolid versus vancomycin for the treatment of MRSA infections in four western European countries [J].
Canut, A. ;
Isla, A. ;
Betriu, C. ;
Gascon, A. R. .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2012, 31 (09) :2227-2235
[8]   Determination of Linezolid in Human Plasma by High-Performance Liquid Chromatography With Ultraviolet Detection [J].
Cattaneo, Dario ;
Baldelli, Sara ;
Conti, Francesca ;
Cozzi, Valeria ;
Clementi, Emilio .
THERAPEUTIC DRUG MONITORING, 2010, 32 (04) :520-524
[9]  
D'Avolio A., 2011, J PHARM BIOMED ANAL, V59, P109
[10]  
D'Avolio A., 2010, J PHARM BIOMED ANAL, V54, P779