Group-specific antibodies against zearalenone and its metabolites and synthetic analogs

被引：15

作者：

Burkin, AA ^{[1
]}

Kononenko, GP ^{[1
]}

Soboleva, NA ^{[1
]}

机构：

[1] Russian Acad Agr Sci, All Russian Res Inst Vet Sanitat Hyg & Ecol, Moscow 123022, Russia

来源：

APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2002年 / 38卷 / 02期

关键词：

D O I：

10.1023/A:1014318818469

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Formation kinetics, specificity, and analytical potential of polyclonal antibodies raised in rabbits against BSA conjugates of zearalanone carboxymethyloxime (CMO-ZAN) and zearalenone carboxymethyloxime (CMO-ZEN) have been studied. Preparation of the conjugates involved conversion of CMO-ZAN and CMO-ZEN into activated esters or carbodiimide condensation. Two versions of a group-specific enzyme immunoassay (for zearalenone/alpha-zearalenol and zearalanone/alpha-zearalanol) based on the heterologous combination of solid-phase antigens are described (sensitivity, 0.01 ng/ml).

引用

页码：169 / 176

页数：8

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