Infectivity of hepatitis C virus correlates with the amount of envelope protein E2: Development of a new aptamer-based assay system suitable for measuring the infectious titer of HCV

被引：40

作者：

Park, Ji Hoon ^{[1
]}

Jee, Min Hyeok ^{[1
]}

Kwon, Oh Sung ^{[1
]}

Keum, Sun Ju ^{[1
]}

Jang, Sung Key ^{[1
,2
]}

机构：

[1] Pohang Univ Sci & Technol, Mol Virol Lab, POSTECH Biotech Ctr, Dept Life Sci, Pohang 790784, Kyungbuk, South Korea

[2] Pohang Univ Sci & Technol, Div Integrat Biosci & Biotechnol, WCU Program, Pohang 790784, Kyungbuk, South Korea

来源：

VIROLOGY | 2013年 / 439卷 / 01期

关键词：

Hepatitis C virus; HCV; Aptamer; SELEX; ELISA; ELASA; Detection; Infectivity measurement; Infectious HCV particles; E2; GLYCOPROTEIN; BINDING; GLYCOSYLATION; ENTRY; CD81; DNA; SENSITIVITY; INTERFERON; PARTICLES; SELECTION;

D O I：

10.1016/j.virol.2013.01.014

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Various forms of hepatitis C virus (HCV)-related particles are produced from HCV-infected cells. Measuring infectivity of a HCV sample with the conventional 'foci counting method' is laborious and time-consuming. Moreover, the infectivity of a HCV sample does not correlate with the amount of viral RNA that can be measured by real-time RT-PCR. Here we report a new assay suitable for quantifying infectious HCV particles using aptamers against HCV E2, which is named 'Enzyme Linked Apto-Sorbent Assay (ELASA)'. The readout value of HCV ELASA linearly correlates with the infectious dose of an HCV sample, but not with the amount of HCV RNA. We also demonstrated that the activities of anti-HCV drugs can be monitored by HCV ELASA. Therefore, HCV ELASA is a quick-and-easy method to quantify infectious units of HCV stocks and to monitor efficacies of potential anti-HCV drugs. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：13 / 22

页数：10

共 61 条

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