A comparative study on subacute toxicity of arsenic trioxide and dimethylarsinic acid on antioxidant status in Crandell Rees feline kidney (CRFK), human hepatocellular carcinoma (PLC/PRF/5), and epithelioma papulosum cyprini (EPC) cell lines

被引：14

作者：

Elia, Antonia Concetta ^{[1
]}

Magara, Gabriele ^{[1
]}

Caruso, Claudio ^{[2
]}

Masoero, Loretta ^{[2
]}

Prearo, Marino ^{[2
]}

Arsieni, Paola ^{[2
]}

Caldaroni, Barbara ^{[1
]}

Dorr, Ambrosius Josef Martin ^{[1
]}

Scoparo, Melissa ^{[1
]}

Salvati, Stefania ^{[2
]}

Brizio, Paola ^{[2
]}

Squadrone, Stefania ^{[2
]}

Abete, Maria Cesarina ^{[2
]}

机构：

[1] Univ Perugia, Dipartimento Chim Biol & Biotecnol, Perugia, Italy

[2] Ist Zooprofilatt Sperimentale Piemonte, Turin, Italy

来源：

JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES | 2018年 / 81卷 / 10期

关键词：

FRESH-WATER FISH; OXIDATIVE STRESS; MONOMETHYLARSONIC ACID; GLUTATHIONE-REDUCTASE; SODIUM ARSENITE; GENE-EXPRESSION; DNA-DAMAGE; INHIBITION; EXPOSURE; APOPTOSIS;

D O I：

10.1080/15287394.2018.1442758

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Arsenic (As) is a global contaminant of terrestrial and aquatic environments posing concern for environmental and human health. The effects of subacute concentrations of arsenic trioxide (As-III) and dimethylarsinic acid (DMA(V)) were examined using Crandell Rees feline kidney (CRFK), human hepatocellular carcinoma (PLC/PRF/5), and epithelioma papulosum cyprini (EPC). Whole monolayer with suffering cells (confluence 100%, pyknosis and refractive cells; value scale=2) led to identification of subacute As concentrations for the three cell lines. The selected As-III concentrations were 1.33 mu M for CRFK and 33.37 mu M for PLC/PRF/5 and EPC, at 48hr time point. The selected DMA(V) concentrations were 0.67mM for PLC/PRF/5, 1.33mM for CRFK, and 2.67mM for EPC for 48hr. Unlike the As-III test, the three cell lines did not exhibit marked susceptibility to DMA(V)-mediated toxicity. Several oxidative stress biomarker levels, directly or indirectly associated with reactive oxygen species (ROS) elimination including superoxide dismutase, catalase, glutathione peroxidases, glutathione reductase, glutathione S-transferase, glyoxalase I, glyoxalase II, and total glutathione, were determined in the three cell lines at 24 and 48hr. Antioxidant responses in metal-treated cells were significantly altered compared to controls, suggesting a perturbation of redox state. The weakening of antioxidant pathway in either healthy or tumoral cells was greater using As-III than DMA(V). Differences in level of several oxidative stress biomarkers suggest that the oxidative stress mechanism induced by As-III is distinctly different from DMA(V). Multifaceted mechanisms of action underlying ROS generation in tumor and nontumor cells versus As-III and DMA(V) exposure are thus involved. Since As-mediated toxicity is quite complex, more data regarding both oxidant-enhancement and oxidant-lowering strategies may be useful to improve knowledge regarding the influence of As on human and animal cells.

引用

页码：333 / 348

页数：16

共 76 条

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