Mechanotransducers in rat pulpal afferents

被引:48
作者
Hermanstyne, T. O. [2 ]
Markowitz, K. [3 ]
Fan, L. [1 ]
Gold, M. S. [1 ]
机构
[1] Univ Pittsburgh, Div Gastroenterol Hepatol & Nutr, Dept Med, Pittsburgh, PA 15213 USA
[2] Univ Maryland, Program Neurosci, Baltimore, MD 21201 USA
[3] Univ Med & Dent New Jersey, New Jersey Dent Sch, Dept Oral Biol, Newark, NJ 07103 USA
关键词
dentin hypersensitivity; single-cell PCR; retrograde labeling; primary afferent;
D O I
10.1177/154405910808700910
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
The hydrodynamic theory suggests that pain associated with stimulation of a sensitive tooth ultimately involves mechanotransduction as a consequence of fluid movement within exposed dentinal tubules. To determine whether putative mechanotransducers could underlie mechanotransduction in pulpal afferents, we used a single-cell PCR approach to screen retrogradely labeled pulpal afferents. The presence of mRNA encoding BNC-1, ASIC3, TRPV4, TRPA1, the alpha, beta, and gamma subunits of ENaC, and the two pore K+ channels (TREK1, TREK2) and TRAAK were screened in pulpal neurons from rats with and without pulpal inflammation. ASIC3, TRPA1, TREK1, and TREK2 were present in similar to 67%, 64%, 14%, and 10% of pulpal neurons, respectively. There was no detectable influence of inflammation on the proportion of neurons expressing these mechanotransducers. Given that the majority of pulpal afferents express ASIC3 and TRPA1, our results raise the possibility that these channels may be novel targets for the treatment of dentin sensitivity.
引用
收藏
页码:834 / 838
页数:5
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