Optimization of Process Parameters for the Production of γ-Linolenic Acid by Cunninghamella elegans CFR C07 in Submerged Fermentation

被引：3

作者：

Varma, Parvathy Sree ^{[1
]}

Chandrasekharan, Salini ^{[1
]}

Venkateswaran, Govindarajulu ^{[2
]}

Rajendran, Santhosh ^{[2
]}

Mallapureddy, Kiran Kumar ^{[1
]}

Pandey, Ashok ^{[1
,3
]}

Parameswaran, Binod ^{[1
]}

机构：

[1] CSIR Natl Inst Interdisciplinary Sci & Technol, Microbial Proc & Technol Div, Ind Estate PO, Thiruvananthapuram 695019, Kerala, India

[2] CSIR Cent Food Technol Res Inst, Mysore 570020, Karnataka, India

[3] CSIR Indian Inst Toxicol Res, 31 MG Marg, Lucknow 226001, Uttar Pradesh, India

来源：

FOOD TECHNOLOGY AND BIOTECHNOLOGY | 2018年 / 56卷 / 01期

关键词：

gamma-linolenic acid; submerged fermentation; Cunninghamella elegans; fatty acid production; EXTRACTION METHODS; GROWTH;

D O I：

10.17113/ftb.56.01.18.5464

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The production of gamma-linolenic acid (GLA) by the fungus Cunninghamella elegans CFR C07 in submerged fermentation was studied. Culture parameters such as carbon source and incubation time were optimized. Four different extraction methods using solvents with acid washed sand, glass beads, lyophilization and Soxhlet extraction were evaluated for improved extraction of lipids from the fungal biomass after fermentation. The GLA production was initially optimized in 250-mL flask and the process was demonstrated in a 3-litre fermentor. The maximum GLA production was 882 mg/L in shake flask culture and 733 mg/L in the fermentor. The study shows that Cunninghamella elegans CFR C07 is a potent organism for the production of GLA under submerged conditions.

引用

页码：96 / 100

页数：5

共 18 条

[1]

Ahmed SU, 2006, FOOD TECHNOL BIOTECH, V44, P283

[2]

Arjuna A., 2014, INT J PHARM BIO SCI, V5, P931

[3] FATTY MATERIAL IN BACTERIA AND FUNGI REVEALED BY STAINING DRIED, FIXED SLIDE PREPARATIONS [J].