Virulence Patterns in a Murine Sepsis Model of ST131 Escherichia coli Clinical Isolates Belonging to Serotypes O25b:H4 and O16:H5 Are Associated to Specific Virotypes

被引:35
作者
Mora, Azucena [1 ]
Dahbi, Ghizlane [1 ]
Lopez, Cecilia [1 ]
Mamani, Rosalia [1 ]
Marzoa, Juan [1 ]
Dion, Sara [2 ,3 ]
Picard, Bertrand [2 ,4 ]
Blanco, Miguel [1 ]
Pilar Alonso, Maria [5 ]
Denamur, Erick [2 ,3 ]
Blanco, Jorge [1 ]
机构
[1] USC, LREC, Dept Microbiol & Parasitoloxia, Fac Vet, Lugo, Spain
[2] INSERM, IAME, UMR 1137, Paris, France
[3] Univ Paris Diderot, Sorbonne Paris Cite, Paris, France
[4] Univ Paris 13, Sorbonne Paris Cite, Paris, France
[5] HULA, Unidade Microbiol Clin, Lugo, Spain
关键词
SEQUENCE TYPE ST131; SPECTRUM BETA-LACTAMASES; EXTENDED-SPECTRUM; MOLECULAR EPIDEMIOLOGY; PHYLOGENETIC GROUP; CLONAL GROUP; EMERGENCE; CTX-M-15; RESISTANCE; STRAINS;
D O I
10.1371/journal.pone.0087025
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Escherichia coli sequence type (ST) 131 is an emerging disseminated public health threat implicated in multidrug-resistant extraintestinal infections worldwide. Although the majority of ST131 isolates belong to O25b:H4 serotype, new variants with different serotypes, STs using the discriminative multilocus sequence typing scheme of Pasteur Institute, and virulence-gene profiles (virotypes) have been reported with unknown implications on the pattern of spread, persistence and virulence. The aim of the present study was to compare virulence in a mouse subcutaneous sepsis model of representative ST131 clinical isolates belonging to 2 serotypes (O25b:H4, O16:H5) and nine virotypes and subtypes (A, B, C, D1, D2, D3, D4, D5 and E). Fourteen out of the 23 ST131 isolates tested (61%) killed 90 to 100% of mice challenged, and 18 of 23 (78%) at least 50%. Interestingly, different virulence patterns in association with virotypes were observed, from highly rapid lethality (death in less than 24 h) to low final lethality (death at 7 days) but with presence of an acute inflammation. This is the first study to assess virulence of ST131 isolates belonging to serotype O16:H5, which exhibited virotype C. In spite of their low virulence-gene score, O16:H5 isolates did not show significant differences in final lethality compared with highly virulent O25b:H4 isolates of virotypes A, B and C, but killed mice less rapidly. Significant differences were found, however, between virotypes A, B, C (final lethality >= 80% of mice challenged) and virotypes D, E. Particularly unexpected was the low lethality of the newly assigned virotype E taking into account that it exhibited high virulence-gene score, and the same clonotype H30 as highly virulent O25b:H4 isolates of virotypes A, B and C. In vivo virulence diversity reported in this study would reflect the genetic variability within ST131 clonal group evidenced by molecular typing.
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页数:9
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