Genotypic comparison between Corynebacterium pseudotuberculosis samples obtained from sheep and goats with caseous lymphadenitis, raised in the semi-arid region of Pernambuco

被引：6

作者：

Abreu, Silvio Romero de O. ^{[1
]}

Mota, Rinaldo A. ^{[2
]}

Rosinha, Gracia Maria S. ^{[3
]}

Forner, Odineia ^{[3
]}

Pinheiro, Jose W., Jr. ^{[2
]}

Pereira, Renata R. B. ^{[3
]}

de Castro, Roberto S. ^{[2
]}

Elisei, Carina ^{[3
]}

Soares, Cleber O. ^{[3
]}

Araujo, Flabio R. ^{[3
]}

Madureira, Renata C. ^{[3
]}

机构：

[1] Ctr Ensino Super Maceio CESMAC, Fac Ciencias Biol & Saude, Clin Escola Med Vet, BR-57160000 Marechal Deodoro, AL, Brazil

[2] Univ Fed Pernambuco, Dept Vet Med, BR-52171900 Recife, PE, Brazil

[3] Empresa Brasileira Pesquisa Agropecuaria, Ctr Nacl Pesquisa Gado Corte, BR-79002970 Campo Grande, MS, Brazil

来源：

PESQUISA VETERINARIA BRASILEIRA | 2008年 / 28卷 / 10期

关键词：

Genotyping; RFLP-PCR; Corynebacterium pseudotuberculosis; goats; sheep;

D O I：

10.1590/S0100-736X2008001000007

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The objective was to genotypically compare 35 samples of Corynebacterium pseudotuberculosis obtained from abscesses of sheep and goats diagnosed with caseous lymphadenitis originated from 5 different municipalities in the semi-arid region of Pernambuco, Brazil. The RFLP-PCR technique with Hpy-Ch4 and Msp I and Pst I Msp I restriction enzimes was used to fingerprint the genes rpoB and pld, respectively. The results demonstrate that there was no difference on the fragments banding pattern among samples, independently of the host species or geographic area studied, defining a homogeneous profile of C. pseudotuberculosis responsible for superficial abscesses for the region.

引用

页码：481 / 487

页数：7

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