An Efficient Light-Driven P450 BM3 Biocatalyst

被引：114

作者：

Ngoc-Han Tran ^{[1
]}

Daniel Nguyen ^{[1
]}

Dwaraknath, Sudharsan ^{[1
]}

Mahadevan, Sruthi ^{[1
]}

Chavez, Garrett ^{[1
]}

Angelina Nguyen ^{[1
]}

Thanh Dao ^{[1
]}

Mullen, Sarah ^{[1
]}

Thien-Anh Nguyen ^{[1
]}

Cheruzel, Lionel E. ^{[1
]}

机构：

[1] San Jose State Univ, Dept Chem, San Jose, CA 95192 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2013年 / 135卷 / 39期

基金：

美国国家科学基金会;

关键词：

ELECTRON-TRANSFER; CYTOCHROME-P450; ACTIVATION; HYDROXYLATION; CHEMISTRY; CATALYSIS; OXIDATION; CYP102A1; ENZYMES;

D O I：

10.1021/ja409337v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

P450s are heme thiolate enzymes that catalyze the regio- and stereoselective functionalization of unactivated C-H bonds using molecular dioxygen and two electrons delivered; by the reductase. We have developed hybrid P450 BM3 heme domains containing a covalently attached Ru(II) photosensitizer in order to circumvent the dependency on the reductase and perform P450 reactions upon visible light irradiation. A highly active hybrid enzyme with improved stability and a modified Ru(II) photosensitizer is able to catalyze the light driven hydroxylation of lauric acid with total turnover numbers of 935 and initial reaction rate of 125 mol product/(mol enzyme/miri).

引用

页码：14484 / 14487

页数：4

共 49 条

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