Hacking the Cancer Genome: Profiling Therapeutically Actionable Long Non-coding RNAs Using CRISPR-Cas9 Screening

被引：164
作者：
Esposito, Roberta [1 ,2 ]
Bosch, Nuria [1 ,2 ,3 ]
Lanzos, Andres [1 ,2 ,3 ]
Polidori, Taisia [1 ,2 ,3 ]
Pulido-Quetglas, Carlos [1 ,2 ,3 ]
Johnson, Rory [1 ,2 ]
机构：
[1] Univ Bern, Bern Univ Hosp, Dept Med Oncol, Inselspital, Bern, Switzerland
[2] Univ Bern, Dept BioMed Res, Bern, Switzerland
[3] Univ Bern, Grad Sch Cellular & Biomed Sci, Bern, Switzerland
来源：
CANCER CELL | 2019年 / 35卷 / 04期
基金：
瑞士国家科学基金会;
关键词：
TRANSCRIPTIONAL ACTIVATION; CHROMATIN-STATE; GENE-EXPRESSION; EMERGING ROLE; LARGE-SCALE; LNCRNA; DATABASE; HALLMARKS; DELETION; ELEMENTS;
D O I：
10.1016/j.ccell.2019.01.019
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Long non-coding RNAs (lncRNAs) represent a huge reservoir of potential cancer targets. Such "onco-lncRNAs" have resisted traditanal RNAi methods, but CRIrPR-Cas9 genome editing now promises functional screens at high throughput and low cost. The unique biology of lncRNAs demands screening strategies distinct from protein-coding genes. The first such screens have identified hundreds of onco-lncRNAs promoting cell proliferation and drug resistance. Ongoing developments will further improve screen performance and translational relevance. This Review aims to highlight the potential of CRISPR screening technology for discovering new onco-lncRNAs, and to guide molecular oncologists wishing to apply it to their cancer of interest.
引用
收藏
页码：545 / 557
页数：13
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