A genetically encoded 19F NMR probe for lysine acetylation

被引：50

作者：

Zhang, Feng ^{[1
,2
,3
,4
]}

Zhou, Qing ^{[2
]}

Yang, Guiwen ^{[1
]}

An, Liguo ^{[1
]}

Li, Fahui ^{[2
]}

Wang, Jiangyun ^{[2
]}

机构：

[1] Shandong Normal Univ, Coll Life Sci, Shandong Prov Key Lab Anim Resistance Biol, 88 East Wenhua Rd, Jinan 250014, Shandong, Peoples R China

[2] Chinese Acad Sci, Inst Biophys, Lab RNA Biol, 15 Datun Rd, Beijing 100101, Peoples R China

[3] Taishan Med Univ, Key Lab Atherosclerosis Univ Shandong, Tai An 271000, Shandong, Peoples R China

[4] Taishan Med Univ, Inst Atherosclerosis, Tai An 271000, Shandong, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2018年 / 54卷 / 31期

基金：

美国国家科学基金会;

关键词：

UNNATURAL AMINO-ACIDS; HISTONE ACETYLATION; CHROMATIN-STRUCTURE; MAMMALIAN-CELLS; DNA-BINDING; POSTTRANSLATIONAL MODIFICATION; PHOTOCLICK-CHEMISTRY; PROTEIN INTERACTIONS; CROSS-LINK; P53;

D O I：

10.1039/c7cc09825a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Advances in acetylated protein-protein/DNA interactions depend on the development of a novel NMR (nuclear magnetic resonance) probe to study the conformational changes of acetylated proteins. However, the method for detecting the acetylated protein conformation is underdeveloped. Herein, an acetyllysine mimic has been exploited for detecting the conformational changes of acetylated p53-protein/DNA interactions by genetic code expansion and F-19 NMR. This F-19 NMR probe shows high structural similarity to acetyllysine and could not be deacetylated by sirtuin deacetylase in vitro/vivo. Moreover, acetylation of p53 K164 is reported to be deacetylated by SIRT2 for the first time.

引用

页码：3879 / 3882

页数：4

共 50 条

[41] Spectroscopic study of cyclen-based 19F NMR probe for detection of hydrogen sulfide
Takegami, Shigehiko
Aramoto, Yuki
Konishi, Atsuko
ANALYTICAL SCIENCES, 2022, 38 (05) : 813 - 820
[42] 19F NMR: An underused efficient probe for paramagnetic metal centers in bioinorganic solution chemistry
Belle, Catherine
Beguin, Claude
Hamman, Sylvain
Pierre, Jean-Louis
COORDINATION CHEMISTRY REVIEWS, 2009, 253 (7-8) : 963 - 976
[43] 19F NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells
Ye, Yansheng
Liu, Xiaoli
Zhang, Zeting
Wu, Qiong
Jiang, Bin
Jiang, Ling
Zhang, Xu
Liu, Maili
Pielak, Gary J.
Li, Conggang
CHEMISTRY-A EUROPEAN JOURNAL, 2013, 19 (38) : 12705 - 12710
[44] Difluoromethionine as a novel 19F NMR structural probe for internal amino acid packing in proteins
Vaughan, MD
Cleve, P
Robinson, V
Duewel, HS
Honek, JF
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (37) : 8475 - 8478
[45] Oxygen as a paramagnetic probe of membrane protein structure by cysteine mutagenesis and 19F NMR spectroscopy
Luchette, PA
Prosser, RS
Sanders, CR
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (08) : 1778 - 1781
[46] Difluoromethionine as a novel 19F NMR structural probe for internal amino acid packing in proteins
Department of Chemistry, University of Waterloo, Waterloo, Ont. N2L 3G1, Canada
不详
J Am Chem Soc, 37 (8475-8478):
[47] Use of 19F NMR spectroscopy to probe enzymatic oxidation of fluorine-tagged sulfides
Behrouzian, B
Hodgson, D
Savile, CK
Dawson, B
Buist, PH
Shanklin, J
MAGNETIC RESONANCE IN CHEMISTRY, 2002, 40 (08) : 524 - 528
[48] Parahydrogen-Induced Polarization Transfer to 19F in Perfluorocarbons for 19F NMR Spectroscopy and MRI
Plaumann, Markus
Bommerich, Ute
Trantzschel, Thomas
Lego, Denise
Dillenberger, Sonja
Sauer, Grit
Bargon, Joachim
Buntkowsky, Gerd
Bernarding, Johannes
CHEMISTRY-A EUROPEAN JOURNAL, 2013, 19 (20) : 6334 - 6339
[49] A 19F and 1H goniometric split-millicoil NMR probe for solid-state NMR
Devries, Jeffrey S.
Zhao, Xingang
Sykes, Brian D.
CONCEPTS IN MAGNETIC RESONANCE PART B-MAGNETIC RESONANCE ENGINEERING, 2007, 31B (04) : 203 - 208
[50] Detection of aptamer target binding with 19F NMR
Petersen, Katie
Easdon, Jerry C.
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2014, 247

← 1 2 3 4 5 →