CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen peroxide, and nitric oxide in Arabidopsis thaliana

被引：100

作者：

Zhang, Luosha ^{[1
]}

Shi, Xiong ^{[1
]}

Zhang, Yutao ^{[1
]}

Wang, Jiajing ^{[1
]}

Yang, Jingwei ^{[1
]}

Ishida, Takashi ^{[2
]}

Jiang, Wenqian ^{[1
]}

Han, Xiangyu ^{[1
]}

Kang, Jingke ^{[1
]}

Wang, Xuening ^{[1
]}

Pan, Lixia ^{[1
]}

Lv, Shuo ^{[1
]}

Cao, Bing ^{[1
]}

Zhang, Yonghong ^{[3
]}

Wu, Jinbin ^{[4
]}

Han, Huibin ^{[5
]}

Hu, Zhubing ^{[6
]}

Cui, Langjun ^{[1
]}

Sawa, Shinichiro ^{[7
]}

He, Junmin ^{[1
]}

Wang, Guodong ^{[1
]}

机构：

[1] Shaanxi Normal Univ, Key Lab Med Resources & Nat Pharmaceut Chem, Natl Engn Lab Resource Dev Endangered Crude Drugs, Coll Life Sci,Minist Educ, Xian 710119, Shaanxi, Peoples R China

[2] Kumamoto Univ, IROAST, Kumamoto 8608555, Japan

[3] Hubei Univ Med, Sch Basic Med, Lab Med Plant, Shiyan 442000, Peoples R China

[4] Wageningen Univ, Lab Phytopathol, NL-6708 PB Wageningen, Netherlands

[5] IST Austria, A-3400 Klosterneuburg, Austria

[6] Henan Univ, Inst Plant Stress Biol, Dept Biol, State Key Lab Cotton Biol, Kaifeng 475001, Peoples R China

[7] Kumamoto Univ, Grad Sch Sci & Technol, Kumamoto 8608555, Japan

来源：

PLANT CELL AND ENVIRONMENT | 2019年 / 42卷 / 03期

基金：

中国国家自然科学基金;

关键词：

ABA; CLE peptide; hydrogen peroxide; nitric oxide; stomatal closure; STEM-CELL PROLIFERATION; RECEPTOR KINASE; ROOT-MERISTEM; GENE; EXPRESSION; REVEALS; CLV3; MPK3; CONSUMPTION; GENERATION;

D O I：

10.1111/pce.13475

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

CLE peptides have been implicated in various developmental processes of plants and mediate their responses to environmental stimuli. However, the biological relevance of most CLE genes remains to be functionally characterized. Here, we report that CLE9, which is expressed in stomata, acts as an essential regulator in the induction of stomatal closure. Exogenous application of CLE9 peptides or overexpression of CLE9 effectively led to stomatal closure and enhanced drought tolerance, whereas CLE9 loss-of-function mutants were sensitivity to drought stress. CLE9-induced stomatal closure was impaired in abscisic acid (ABA)-deficient mutants, indicating that ABA is required for CLE9-medaited guard cell signalling. We further deciphered that two guard cell ABA-signalling components, OST1 and SLAC1, were responsible for CLE9-induced stomatal closure. MPK3 and MPK6 were activated by the CLE9 peptide, and CLE9 peptides failed to close stomata in mpk3 and mpk6 mutants. In addition, CLE9 peptides stimulated the induction of hydrogen peroxide (H2O2) and nitric oxide (NO) synthesis associated with stomatal closure, which was abolished in the NADPH oxidase-deficient mutants or nitric reductase mutants, respectively. Collectively, our results reveal a novel ABA-dependent function of CLE9 in the regulation of stomatal apertures, thereby suggesting a potential role of CLE9 in the stress acclimatization of plants.

引用

页码：1033 / 1044

页数：12

共 70 条

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