miR-372 suppresses tumour proliferation and invasion by targeting IGF2BP1 in renal cell carcinoma

被引:69
作者
Huang, Xuan [1 ]
Huang, Mingjie [2 ]
Kong, Lingbao [2 ]
Li, Yong [1 ]
机构
[1] Nanchang Univ, Inst Translat Med, Nanchang 330031, Jiangxi, Peoples R China
[2] Jiangxi Agr Univ, Coll Biosci & Engn, Nanchang 330045, Jiangxi, Peoples R China
关键词
MESENCHYMAL STEM-CELLS; POOR-PROGNOSIS; EXPRESSION; CANCER; MICRORNA-372; IDENTIFICATION; SENESCENCE; RNA;
D O I
10.1111/cpr.12207
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
ObjectivesMicroRNAs (miRNAs) are endogenous small non-coding RNAs that regulate proteins and mRNAs for degradation or translational suppression. Up to now, the role of miR-372 in renal cell carcinoma has remained unknown; in this study, we have aimed to reveal its functional importance in this tumour. Materials and methodsqRT-PCR was performed to measure expression levels of miR-372 in renal cell carcinoma cell lines and tissues. CCK-8 and an invasion assay were performed to measure its functional role. Luciferase assays, qRT-PCR and western blotting were performed to discover miR-372s target gene. ResultsWe demonstrated that miRNA-372 was down-regulated in renal cell carcinoma cell lines and tissue specimens; its over-expression inhibited cell proliferation and invasion. Moreover, we showed that miRNA-372 repressed insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) expression by directly interacting with its putative binding site at the 3-UTR. Furthermore, ectopic expression of IGF2BP1 significantly reversed suppression of cell proliferation and invasion caused by miR-372 over-expression. ConclusionsOur data indicated that miR-372 seemed to function as a tumour suppressor in renal cell carcinoma progression by inhibiting the IGF2BP1 expression.
引用
收藏
页码:593 / 599
页数:7
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