Optimization of the technology of extracting water-soluble polysaccharides from Morus alba L. leaves

To optimize the parameters for extracting water-soluble polysaccharides from mulberry leaves using hot water, the extraction process was optimized by the orthogonal test through the single-factor experiment. Experiments were carried out using an L(9) (3(4)) orthogonal design to examine the effects of extraction temperature, extraction duration, concentration of the material and concentration of ethanol on the polysaccharide yield. The optimum extraction conditions determined were as follows: concentration of material was equal to 1:24, extraction temperature was 70 degrees C, extraction duration was 90 min and concentration of ethanol was equal to 80%. Under these conditions, the yield of polysaccharides was 2.64%.