Rheological Properties of Cross-Linked Hyaluronan-Gelatin Hydrogels for Tissue Engineering

被引：186

作者：

Vanderhooft, Janssen L. ^{[4
]}

Alcoutlabi, Mataz ^{[5
]}

Magda, Jules J. ^{[3
,5
]}

Prestwich, Glenn D. ^{[1
,2
]}

机构：

[1] Univ Utah, Dept Med Chem, Salt Lake City, UT 84108 USA

[2] Univ Utah, Ctr Therapeut Biomat, Salt Lake City, UT 84108 USA

[3] Univ Utah, Dept Chem Engn, Salt Lake City, UT 84108 USA

[4] Univ Utah, Dept Bioengn, Salt Lake City, UT 84108 USA

[5] Univ Utah, Dept Mat Sci & Engn, Salt Lake City, UT 84108 USA

来源：

MACROMOLECULAR BIOSCIENCE | 2009年 / 9卷 / 01期

关键词：

biomaterials; hyaluronic acid; hydrogels; rheology; tissue engineering; MULTIPLE-PARTICLE-TRACKING; SYNTHETIC EXTRACELLULAR-MATRIX; ELASTIC PROPERTIES; YOUNGS MODULUS; POISSONS RATIO; CELL-CULTURE; MICRORHEOLOGY; ELASTOGRAPHY; SCAFFOLD; ADHESIONS;

D O I：

10.1002/mabi.200800141

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydrogels that mimic the natural extracellular matrix (ECM) are used in three-dimensional cell culture, cell therapy, and tissue engineering. A semi-synthetic ECM based on cross-linked hyaluronana offers experimental control of both composition and gel stiffness. The mechanical properties of the ECM in part determine the ultimate cell phenotype. We now describe a rheological study of synthetic ECM hydrogels with storage shear moduli that span three orders of magnitude, from 11 to 3 500 Pa, a range important for engineering of soft tissues. The concentration of the chemically modified HA and the cross-linking density were the main determinants of gel stiffness. Increase in the ratio of thiol-modified gelatin reduced gel stiffness by diluting the effective concentration of the HA component.

引用

页码：20 / 28

页数：9

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