Application of denaturing gradient gel electrophoresis for detection of bacterial and yeast communities along a salinity gradient in the estuary of the Cachoeira River in Brazil

被引：6

作者：

Rodrigues, C. S. P. ^{[1
]}

Souza, S. S. ^{[1
]}

Rezende, R. P. ^{[2
]}

Silva, A. ^{[2
]}

Andrioli, J. L. ^{[1
]}

Costa, H. ^{[2
]}

Fontana, R. ^{[1
]}

Dias, J. C. T. ^{[1
]}

机构：

[1] Univ Estadual Santa Cruz, Dept Ciencias Biol, Lab Monitoramento Ambiental, Ilheus, BA, Brazil

[2] Univ Estadual Santa Cruz, Dept Ciencias Biol, Lab Biotecnol Microbiana, Ilheus, BA, Brazil

来源：

GENETICS AND MOLECULAR RESEARCH | 2013年 / 12卷 / 02期

关键词：

Microbial community; Estuary; Salinity; DGGE; TIETE RIVER; NITROGEN; DENITRIFICATION; RESERVOIRS;

D O I：

10.4238/2013.May.21.6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An estuary is a transition zone between freshwater and marine ecosystems, resulting in dilution of seawater. Estuaries are also considered environments of intense biological activity related to the processes of nutrient cycling. The aim of this study was to evaluate the microbial community composition along a salinity gradient in the estuary of the Cachoeira River, located in southern Bahia, Brazil. The analysis of bacterial and yeast communities was performed by determining the denaturing gradient gel electrophoresis band richness. Formation of zones with similar profiles of bands was observed, and the increasing richness at the intermediate zone demonstrated a clear spatial distinction of communities depending on salinity. In addition, the dissolved oxygen content, temperature, pH, salinity, and dissolved inorganic nutrient contents (NH3+, NO2-, NO3-, PO4-) were determined. Nutrients were distributed in similar patterns, with decreasing concentrations as the salinity increases.

引用

页码：1752 / 1760

页数：9

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