Long noncoding RNA PVT1 promotes EMT via mediating microRNA-186 targeting of Twist1 in prostate cancer

被引：82

作者：

Chang, Zhongwen ^{[1
]}

Cui, Jun ^{[1
]}

Song, Yongsheng ^{[1
]}

机构：

[1] China Med Univ, Shengjing Hosp, Dept Urol, Shenyang 110004, Liaoning, Peoples R China

来源：

GENE | 2018年 / 654卷

关键词：

Prostate cancer; EMT; lncRNA; Twist; PVT1; Survival outcomes; METASTASIS; BIOMARKER;

D O I：

10.1016/j.gene.2018.02.036

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The pathogenesis and the underlying mechanism of endothelial-mesenchymal transition in prostate cancer remain unclear. Plasmacytoma variant translocation 1 (PVT1), a novel long non-coding RNA maps to 8q24.21, and in many tumor studies the up-regulation of PVT1 has already been reported. PVT1 is closely related to tumor cell proliferation, invasion, and metastasis. In this study, we employed a combination of techniques to study the role of PVT1 in prostate cancer, which included bioinformatic analysis, Western blotting and cell migration assays of prostate cancer cell lines. We report that PVT1 promotes prostate cancer invasion and metastasis by modulating EMT. Furthermore, PVT1 can promote EMT by up-regulation of Twist1, a transcription factor associated with EMT. We then confirmed that PVT1 acts as a sponge for miRNA-186-5p and positively regulates Twist1 by a sponge effect. Therefore, this study has revealed a novel MECHANISM for the promotion of EMT in prostate cancer by PVT1. Our findings suggest that the PVT1/miR-186/Twist1 regulatory axis may be a new therapeutic target for prostate cancer.

引用

页码：36 / 42

页数：7

共 23 条

[1] MALAT1 and HOTAIR Long Non-Coding RNAs Play Opposite Role in Estrogen-Mediated Transcriptional Regulation in Prostate Cancer Cells [J].

Aiello, Aurora ;

Bacci, Lorenza ;

Re, Agnese ;

Ripoli, Cristian ;

Pierconti, Francesco ;

Pinto, Francesco ;

Masetti, Riccardo ;

Grassi, Claudio ;

Gaetano, Carlo ;

Bassi, Pier Francesco ;

Pontecorvi, Alfredo ;

Nanni, Simona ;

Farsetti, Antonella .

SCIENTIFIC REPORTS, 2016, 6

[2] Role of the long non-coding RNA PVT1 in the dysregulation of the ceRNA-ceRNA network in human breast cancer [J].

Conte, Federica ;

Fiscon, Giulia ;

Chiara, Matteo ;

Colombo, Teresa ;

Farina, Lorenzo ;

Paci, Paola .

PLOS ONE, 2017, 12 (02)

[3] Persistent androgen receptor-mediated transcription in castration-resistant prostate cancer under androgen-deprived conditions [J].

Decker, Keith F. ;

Zheng, Dali ;

He, Yuhong ;

Bowman, Tamara ;

Edwards, John R. ;

Jia, Li .

NUCLEIC ACIDS RESEARCH, 2012, 40 (21) :10765-10779

[4] Androgen deprivation therapy: progress in understanding mechanisms of resistance and optimizing androgen depletion [J].

Harris, William P. ;

Mostaghel, Elahe A. ;

Nelson, Peter S. ;

Montgomery, Bruce .

NATURE CLINICAL PRACTICE UROLOGY, 2009, 6 (02) :76-85

[5] Long Non-Coding RNAs: Key Regulators of Epithelial-Mesenchymal Transition, Tumour Drug Resistance and Cancer Stem Cells [J].

Heery, Richard ;

Finn, Stephen P. ;

Cuffe, Sinead ;

Gray, Steven G. .

CANCERS, 2017, 9 (04)

[6] PVT1 Exon 9: A Potential Biomarker of Aggressive Prostate Cancer? [J].

Ilboudo, Adeodat ;

Chouhan, Jyoti ;

McNeil, Brian K. ;

Osborne, Joseph R. ;

Ogunwobi, Olorunseun O. .

INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, 2016, 13 (01)

[7] Abiraterone for Prostate Cancer Not Previously Treated with Hormone Therapy [J].

James, N. D. ;

de Bono, J. S. ;

Spears, M. R. ;

Clarke, N. W. ;

Mason, M. D. ;

Dearnaley, D. P. ;

Ritchie, A. W. S. ;

Amos, C. L. ;

Gilson, C. ;

Jones, R. J. ;

Matheson, D. ;

Miliman, R. ;

Attard, G. ;

Chowdhury, S. ;

Cross, W. R. ;

Gillessen, S. ;

Parker, C. C. ;

Russell, J. M. ;

Berthold, D. R. ;

Brawley, C. ;

Adab, F. ;

Aung, S. ;

Birtle, A. J. ;

Bowen, J. ;

Brock, S. ;

Chakraborti, P. ;

Ferguson, C. ;

Gale, J. ;

Gray, E. ;

Hingorani, M. ;

Hoskin, P. J. ;

Lester, J. F. ;

Malik, Z. I. ;

McKinna, F. ;

McPhail, N. ;

Money-Kyrle, J. ;

O'Sullivan, J. ;

Parikh, O. ;

Protheroe, A. ;

Robinson, A. ;

Srihari, N. N. ;

Thomas, C. ;

Wagstaff, J. ;

Wylie, J. ;

Zarkar, A. ;

Parmar, M. K. B. ;

Sydes, M. R. .

NEW ENGLAND JOURNAL OF MEDICINE, 2017, 377 (04) :338-351

[8] Long Noncoding RNAs as Putative Biomarkers for Prostate Cancer Detection [J].

Lee, Bongyong ;

Mazar, Joseph ;

Aftab, Muhammad N. ;

Qi, Feng ;

Shelley, John ;

Li, Jian-Liang ;

Govindarajan, Subramaniam ;

Valerio, Felipe ;

Rivera, Inoel ;

Thurn, Tadzia ;

Tien Anh Tran ;

Kameh, Darian ;

Patel, Vipul ;

Perera, Ranjan J. .

JOURNAL OF MOLECULAR DIAGNOSTICS, 2014, 16 (06) :615-626

[9] Contributions of epithelial-mesenchymal transition and cancer stem cells to the development of castration resistance of prostate cancer [J].

Li, Ping ;

Yang, Ru ;

Gao, Wei-Qiang .

MOLECULAR CANCER, 2014, 13

[10] Long Noncoding RNA-LET Suppresses Tumor Growth and EMT in Lung Adenocarcinoma [J].

Liu, Bin ;

Pan, Chun-Feng ;

He, Zhi-Cheng ;

Wang, Jun ;

Wang, Peng-Li ;

Ma, Teng ;

Xia, Yang ;

Chen, Yi-Jiang .

BIOMED RESEARCH INTERNATIONAL, 2016, 2016

← 1 2 3 →