High-yield isolation of extracellular vesicles using aqueous two-phase system

被引：113

作者：

Shin, Hyunwoo ^{[1
]}

Han, Chungmin ^{[1
]}

Labuz, Joseph M. ^{[4
]}

Kim, Jiyoon ^{[2
]}

Kim, Jongmin ^{[1
]}

Cho, Siwoo ^{[1
]}

Gho, Yong Song ^{[3
]}

Takayama, Shuichi ^{[4
,5
]}

Park, Jaesung ^{[1
,2
]}

机构：

[1] POSTECH, Dept Mech Engn, Pohang 790784, Gyeongbuk, South Korea

[2] POSTECH, Sch Interdisciplinary Biosci & Bioengn, Pohang 790784, Gyeongbuk, South Korea

[3] POSTECH, Dept Life Sci, Pohang 790784, Gyeongbuk, South Korea

[4] Univ Michigan, Dept Biomed Engn, Coll Engn, Biointerfaces Inst, Ann Arbor, MI 48109 USA

[5] Univ Michigan, Coll Engn, Macromol Sci & Engn Ctr, Biointerfaces Inst, Ann Arbor, MI 48109 USA

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

新加坡国家研究基金会;

关键词：

CELL-DERIVED EXOSOMES; INTERSTITIAL FLUID; SEPARATION; ANTIGEN; PROTEIN; MICROENVIRONMENT; BIOMARKERS; PARTITION; MICRORNA; LIQUID;

D O I：

10.1038/srep13103

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs as diagnostic biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne EVs have been hindered by the lack of effective purification strategies. In this study, an efficient EV isolation method was developed by using polyethylene glycol/dextran aqueous two phase system (ATPS). This method provides high EV recovery efficiency (similar to 70%) in a short time (similar to 15 min). Consequently, it can significantly increase the diagnostic applicability of EVs.

引用

页数：11

共 38 条

[1] PARTICLE FRACTIONATION IN LIQUID 2-PHASE SYSTEMS - THE COMPOSITION OF SOME PHASE SYSTEMS AND THE BEHAVIOUR OF SOME MODEL PARTICLES IN THEM APPLICATION TO THE ISOLATION OF CELL WALLS FROM MICROORGANISMS
ALBERTSSON, PA
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1958, 27 (02) : 378 - 395
[2] Comparison of protein, microRNA, and mRNA yields using different methods of urinary exosome isolation for the discovery of kidney disease biomarkers
Alvarez, M. Lucrecia
Khosroheidari, Mahdieh
Ravi, Rupesh Kanchi
DiStefano, Johanna K.
[J]. KIDNEY INTERNATIONAL, 2012, 82 (09) : 1024 - 1032
[3] Arneson W., 2007, Clinical Chemistry - A Laboratory Perspective
[4] ATHA DH, 1981, J BIOL CHEM, V256, P2108
[5] Proteomic characterization of the interstitial fluid perfusing the breast tumor microenvironment -: A novel resource for biomarker and therapeutic target discovery
Celis, JE
Gromov, P
Cabezón, T
Moreira, JMA
Ambartsumian, N
Sandelin, K
Rank, F
Gromova, I
[J]. MOLECULAR & CELLULAR PROTEOMICS, 2004, 3 (04) : 327 - 344
[6] Microfluidic isolation and transcriptome analysis of serum microvesicles
Chen, Chihchen
Skog, Johan
Hsu, Chia-Hsien
Lessard, Ryan T.
Balaj, Leonora
Wurdinger, Thomas
Carter, Bob S.
Breakefield, Xandra O.
Toner, Mehmet
Irimia, Daniel
[J]. LAB ON A CHIP, 2010, 10 (04) : 505 - 511
[7] Analysis of antigen presenting cell derived exosomes, based on immuno-magnetic isolation and flow cytometry
Clayton, A
Court, J
Navabi, H
Adams, M
Mason, MD
Hobot, JA
Newman, GR
Jasani, B
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 2001, 247 (1-2) : 163 - 174
[8] CD45 immunoaffinity depletion of vesicles from Jurkat T cells demonstrates that exosomes contain CD45: no evidence for a distinct exosome/HIV-I budding pathway
Coren, Lori V.
Shatzer, Teresa
Ott, David E.
[J]. RETROVIROLOGY, 2008, 5 (1)
[9] Tumor-derived microvesicles: shedding light on novel microenvironment modulators and prospective cancer biomarkers
D'Souza-Schorey, Crislyn
Clancy, James W.
[J]. GENES & DEVELOPMENT, 2012, 26 (12) : 1287 - 1299
[10] Microfluidic filtration system to isolate extracellular vesicles from blood
Davies, Ryan T.
Kim, Junho
Jang, Su Chul
Choi, Eun-Jeong
Gho, Yong Song
Park, Jaesung
[J]. LAB ON A CHIP, 2012, 12 (24) : 5202 - 5210

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