Evaluation of bioactivity of octacalcium phosphate using osteoblastic cell aggregates on a spheroid culture device

被引:9
作者
Anada, Takahisa [1 ]
Sato, Tomoya [1 ,2 ]
Kamoya, Takuo [1 ,3 ]
Shiwaku, Yukari [1 ,4 ]
Tsuchiya, Kaori [1 ]
Takano-Yamamoto, Teruko [3 ]
Sasaki, Keiichi [2 ]
Suzuki, Osamu [1 ]
机构
[1] Tohoku Univ, Grad Sch Dent, Div Craniofacial Funct Engn, Sendai, Miyagi, Japan
[2] Tohoku Univ, Grad Sch Dent, Div Adv Prosthet Dent, Sendai, Miyagi, Japan
[3] Tohoku Univ, Grad Sch Dent, Div Orthodont & Dentofacial Orthoped, Sendai, Miyagi, Japan
[4] Tohoku Univ, Grad Sch Dent, Liaison Ctr Innovat Dent, Sendai, Miyagi, Japan
来源
REGENERATIVE THERAPY | 2016年 / 3卷
关键词
Spheroids; Calcium phosphate; Octacalcium phosphate; Oxygen-permeable chip; Osteoblastic differentiation; BONE-FORMATION; STEM-CELLS; HYDROXYAPATITE; CHEMISTRY; SCAFFOLD;
D O I
10.1016/j.reth.2016.02.004
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Much attention has been paid to three-dimensional cell culture systems in the field of regenerative medicine, since three-dimensional cellular aggregates, or spheroids, are thought to better mimic the in vivo microenvironments compared to conventional monolayer cultured cells. Synthetic calcium phosphate (CaP) materials are widely used as bone substitute materials in orthopedic and dental surgeries. Here we have developed a technique for constructing a hybrid spheroid consisting of mesenchymal stem cells (MSCs) and synthetic CaP materials using a spheroid culture device. We found that the device is able to generate uniform-sized CaP/cell hybrid spheroids rapidly and easily. The results showed that the extent of osteoblastic differentiation from MSCs was different when cells were grown on octacalcium phosphate (OCP), hydroxyapatite (HA), or beta-tricalcium phosphate (beta-TCP). OCP showed the greatest ability to increase the alkaline phosphatase activity of the spheroid cells. The results suggest that the spheroids with incorporated OCP may be an effective implantable hybrid consisting of scaffold material and cells for bone regeneration. It is also possible that this CaP-cell spheroid system may be used as an in vitro method for assessing the osteogenic induction ability of CaP materials. (c) 2016, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.
引用
收藏
页码:58 / 62
页数:5
相关论文
共 18 条
  • [1] Anada T, 2008, TISSUE ENG PT A, V14, P965, DOI [10.1089/ten.tea.2007.0339, 10.1089/tea.2007.0339]
  • [2] An oxygen-permeable spheroid culture system for the prevention of central hypoxia and necrosis of spheroids
    Anada, Takahisa
    Fukuda, Junji
    Sai, Yuko
    Suzuki, Osamu
    [J]. BIOMATERIALS, 2012, 33 (33) : 8430 - 8441
  • [3] Three-dimensional cell culture device utilizing thin membrane deformation by decompression
    Anada, Takahisa
    Masuda, Taisuke
    Honda, Yoshitomo
    Fukuda, Junji
    Arai, Fumihito
    Fukuda, Toshio
    Suzuki, Osamu
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 2010, 147 (01) : 376 - 379
  • [4] CRYSTAL-CHEMISTRY OF OCTACALCIUM PHOSPHATE
    BROWN, WE
    MATHEW, M
    TUNG, MS
    [J]. PROGRESS IN CRYSTAL GROWTH AND CHARACTERIZATION OF MATERIALS, 1981, 4 (1-2) : 59 - 87
  • [5] Chow LC, 2009, DENT MATER J, V28, P1
  • [6] Osteogenic differentiation of mouse embryonic stem cells and mouse embryonic fibroblasts in a three-dimensional self-assembling peptide scaffold
    Garreta, Elena
    Genove, Elsa
    Borros, Salvador
    Semino, Carlos E.
    [J]. TISSUE ENGINEERING, 2006, 12 (08): : 2215 - 2227
  • [7] Preparation of stem cell aggregates with gelatin microspheres to enhance biological functions
    Hayashi, Kentaro
    Tabata, Yasuhiko
    [J]. ACTA BIOMATERIALIA, 2011, 7 (07) : 2797 - 2803
  • [8] THE EFFECT OF SYNTHETIC OCTACALCIUM PHOSPHATE IN A COLLAGEN SCAFFOLD ON THE OSTEOGENICITY OF MESENCHYMAL STEM CELLS
    Kawai, Tadashi
    Anada, Takahisa
    Masuda, Taisuke
    Honda, Yoshitomo
    Sakai, Yuhiro
    Kato, Yukio
    Kamakura, Shinji
    Echigo, Seishi
    Suzuki, Osamu
    [J]. EUROPEAN CELLS & MATERIALS, 2011, 22 : 124 - 136
  • [9] Fabrication of microchannel networks in multicellular spheroids
    Kojima, Nobuhiko
    Takeuchi, Shoji
    Sakai, Yasuyuki
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 2014, 198 : 249 - 254
  • [10] Effect of partial hydrolysis of octacalcium phosphate on its osteoconductive characteristics
    Miyatake, Naohisa
    Kishimoto, Koshi N.
    Anada, Takahisa
    Imaizumi, Hideki
    Itoi, Eiji
    Suzuki, Osamu
    [J]. BIOMATERIALS, 2009, 30 (06) : 1005 - 1014