Evaluation of 2,6-dihydroxyacetophenone as matrix-assisted laser desorption/ionization matrix for analysis of hydrophobic proteins and peptides

被引：7

作者：

Jacksen, Johan ^{[1
]}

Emmer, Asa ^{[1
]}

机构：

[1] KTH Royal Inst Technol, Sch Chem Sci & Engn, Dept Chem, SE-10044 Stockholm, Sweden

来源：

ANALYTICAL BIOCHEMISTRY | 2012年 / 425卷 / 01期

基金：

瑞典研究理事会;

关键词：

DHAP; Hydrophobic protein/peptides; MALDI-MS; Matrix; FLIGHT MASS-SPECTROMETRY; MS ANALYSIS; TARGET;

D O I：

10.1016/j.ab.2012.02.039

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is widely used for analysis of macromolecules like peptides and proteins. The analysis procedure is generally simple but must be adapted to the characteristics of the analytes. Therefore, specific matrices suitable for, e.g., hydrophobic proteins and peptides that are difficult to analyze would be preferable in order to optimize the outcome. In the present work, 2,6-dihydroxyacetophenone (DHAP) was shown to be beneficial in comparison to DHB for intact bacteriorhodopsin (BR) as well as for chemically digested BR. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：18 / 20

页数：3

共 16 条

[1] Analysis of quaternary protein ensembles by matrix assisted laser desorption/ionization mass spectrometry [J].

Cohen, LRH ;

Strupat, K ;

Hillenkamp, F .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1997, 8 (10) :1046-1052

[2] Influence of matrix solution conditions on the MALDI-MS analysis of peptides and proteins [J].

Cohen, SL ;

Chait, BT .

ANALYTICAL CHEMISTRY, 1996, 68 (01) :31-37

[3] Hydrophobic peptide mapping of clinically relevant heptahelical membrane proteins by capillary electrophoresis [J].

Dong, MQ ;

Oda, RP ;

Strausbauch, MA ;

Wettstein, PJ ;

Landers, JP ;

Miller, LJ .

ELECTROPHORESIS, 1997, 18 (10) :1767-1774

[4] Identification of tryptophan oxidation products in bovine α-crystallin [J].

Finley, EL ;

Dillon, J ;

Crouch, RK ;

Schey, KL .

PROTEIN SCIENCE, 1998, 7 (11) :2391-2397

[5] MALDI/MS peptide mass fingerprinting for proteome analysis: Identification of hydrophobic proteins attached to eucaryote keratinocyte cytoplasmic membrane using different matrices in concert [J].

Florence Gonnet ;

Gilles Lemaître ;

Gilles Waksman ;

Jeanine Tortajada .

Proteome Science, 1 (1)

[6] Capillary electrophoretic separation and fractionation of hydrophobic peptides onto a pre-structured matrix assisted laser desorption/ionization target for mass spectrometric analysis [J].

Jacksén, J ;

Redeby, T ;

Emmer, Å .

JOURNAL OF SEPARATION SCIENCE, 2006, 29 (02) :288-295

[7] MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS-SPECTROMETRY WITH 2-(4-HYDROXYPHENYLAZO)BENZOIC ACID MATRIX [J].

JUHASZ, P ;

COSTELLO, CE ;

BIEMANN, K .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1993, 4 (05) :399-409

[8] The combination of simple MALDI matrices for the improvement of intact glycoproteins and glycans analysis [J].

Lastovickova, Marketa ;

Chmelik, Josef ;

Bobalova, Janette .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2009, 281 (1-2) :82-88

[9] Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in clinical chemistry [J].

Marvin, LF ;

Roberts, MA ;

Fay, LB .

CLINICA CHIMICA ACTA, 2003, 337 (1-2) :11-21

[10] Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry in the subunit stoichiometry study of high-mass non-covalent complexes [J].

Moniatte, M ;

Lesieur, C ;

Vecsey-Semjen, B ;

Buckley, JT ;

Pattus, F ;

van der Goot, FG ;

Van Dorsselaer, A .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 1997, 169 :179-199

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