OsPFA-DSP1, a rice protein tyrosine phosphatase, negatively regulates drought stress responses in transgenic tobacco and rice plants

被引：25

作者：

Liu, Bing ^{[1
,2
]}

Fan, Jieqiong ^{[1
,2
]}

Zhang, Yang ^{[1
,2
]}

Mu, Peiqiang ^{[1
,2
]}

Wang, Peng ^{[1
,2
]}

Su, Jianbin ^{[1
,2
]}

Lai, Huihuang ^{[1
,2
]}

Li, Shaowu ^{[1
,2
]}

Feng, Dongru ^{[1
,2
]}

Wang, Jinfa ^{[1
,2
]}

Wang, Hongbin ^{[1
,2
]}

机构：

[1] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Key Lab Gene Engn,Minist Educ, Guangzhou 510275, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Sch Life Sci, Guangdong Key Lab Plant Resources, Guangzhou 510275, Guangdong, Peoples R China

来源：

PLANT CELL REPORTS | 2012年 / 31卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Protein tyrosine phosphatase; PFA-DSPs; Drought stress; OsPFA-DSP1; ABSCISIC-ACID; KINASE PHOSPHATASE; ARABIDOPSIS; TOLERANCE; GENE; LOCALIZATION; SPECIFICITY; EXPRESSION; SEQUENCE; SALINITY;

D O I：

10.1007/s00299-011-1220-x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Dephosphorylation plays a pivotal role in regulating plant growth, development and abiotic/biotic stress responses. Here, we characterized a plant and fungi atypical dual-specificity phosphatase (PFA-DSP) subfamily member, OsPFA-DSP1, from rice. OsPFA-DSP1 was determined to be a functional protein tyrosine phosphatase (PTP) in vitro using phosphatase activity assays. Quantitative real-time PCR and GENEVESTIGATOR analysis showed that - mRNA was induced by drought stress. Transfection of rice protoplasts showed that OsPFA-DSP1 accumulated in both the cytoplasm and nucleus. Ectopic overexpression of - in tobacco increased sensitivity to drought stress and insensitivity to ABA-induced stomatal closure and inhibition of stomatal opening. Furthermore, overexpression of - in rice also increased sensitivity to drought stress. These results indicated that OsPFA-DSP1 is a functional PTP and may act as a negative regulator in drought stress responses.

引用

页码：1021 / 1032

页数：12

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