Landscape of transcription in human cells

被引：3940

作者：

Djebali, Sarah ^{[1
,2
]}

Davis, Carrie A. ^{[3
]}

Merkel, Angelika ^{[1
,2
]}

Dobin, Alex ^{[3
]}

Lassmann, Timo ^{[4
]}

Mortazavi, Ali ^{[5
,6
]}

Tanzer, Andrea ^{[1
,2
]}

Lagarde, Julien ^{[1
,2
]}

Lin, Wei ^{[3
]}

Schlesinger, Felix ^{[3
]}

Xue, Chenghai ^{[3
]}

Marinov, Georgi K. ^{[5
]}

Khatun, Jainab ^{[7
]}

Williams, Brian A. ^{[5
]}

Zaleski, Chris ^{[3
]}

Rozowsky, Joel ^{[8
,9
]}

Roeder, Maik ^{[1
,2
]}

Kokocinski, Felix ^{[10
]}

Abdelhamid, Rehab F. ^{[4
]}

Alioto, Tyler ^{[1
,2
,11
]}

Antoshechkin, Igor ^{[5
]}

Baer, Michael T. ^{[3
]}

Bar, Nadav S. ^{[12
]}

Batut, Philippe ^{[3
]}

Bell, Kimberly ^{[3
]}

Bell, Ian ^{[13
]}

Chakrabortty, Sudipto ^{[3
]}

Chen, Xian ^{[14
]}

Chrast, Jacqueline ^{[15
]}

Curado, Joao ^{[1
,2
]}

Derrien, Thomas ^{[1
,2
]}

Drenkow, Jorg ^{[3
]}

Dumais, Erica ^{[13
]}

Dumais, Jacqueline ^{[13
]}

Duttagupta, Radha ^{[13
]}

Falconnet, Emilie ^{[16
,17
]}

Fastuca, Meagan ^{[3
]}

Fejes-Toth, Kata ^{[3
]}

Ferreira, Pedro ^{[1
,2
]}

Foissac, Sylvain ^{[13
]}

Fullwood, Melissa J. ^{[18
]}

Gao, Hui ^{[13
]}

Gonzalez, David ^{[1
,2
]}

Gordon, Assaf ^{[3
]}

Gunawardena, Harsha ^{[14
]}

Howald, Cedric ^{[15
]}

Jha, Sonali ^{[3
]}

Johnson, Rory ^{[1
,2
]}

Kapranov, Philipp ^{[13
,19
]}

King, Brandon ^{[1
,2
,5
]}

机构：

[1] Ctr Gen Regulat, Barcelona 08003, Catalonia, Spain

[2] UPF, Barcelona 08003, Catalonia, Spain

[3] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11742 USA

[4] RIKEN Omics Sci Ctr, RIKEN Yokohama Inst, Tsurumi ku, Yokohama, Kanagawa 2300045, Japan

[5] CALTECH, Beckman Inst 2, Div Biol, Pasadena, CA 91125 USA

[6] Univ Calif Irvine, Dept Dev & Cell Biol, Irvine, CA 92697 USA

[7] Boise State Univ, Coll Arts & Sci, Boise, ID 83725 USA

[8] Yale Univ, Program Computat Biol & Bioinformat, New Haven, CT 06520 USA

[9] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

[10] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England

[11] CNAG, Barcelona 08028, Catalonia, Spain

[12] Norwegian Univ Sci & Technol, Dept Chem Engn, NO-7491 Trondheim, Norway

[13] Affymetrix Inc, Santa Clara, CA 95051 USA

[14] Univ N Carolina, Dept Biochem & Biophys, Chapel Hill, NC 27599 USA

[15] Univ Lausanne, Ctr Integrat Gen, CH-1015 Lausanne, Switzerland

[16] Univ Geneva, Sch Med, Dept Genet Med & Dev, CH-1211 Geneva, Switzerland

[17] IGE3 Inst Genet & Genom Geneva, CH-1211 Geneva, Switzerland

[18] Genome Inst Singapore, Singapore 138672, Singapore

[19] St Laurent Inst, Cambridge, MA 02141 USA

[20] Yale Univ, Dept Comp Sci, New Haven, CT 06520 USA

[21] Univ Pompeu Fabra, Dept Ciencies Expt & Salut, Barcelona, Catalonia, Spain

来源：

NATURE | 2012年 / 489卷 / 7414期

基金：

欧洲研究理事会; 美国国家科学基金会;

关键词：

RNA; GENOME; IDENTIFICATION; ENHANCERS; MAPS;

D O I：

10.1038/nature11233

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Eukaryotic cells make many types of primary and processed RNAs that are found either in specific subcellular compartments or throughout the cells. A complete catalogue of these RNAs is not yet available and their characteristic subcellular localizations are also poorly understood. Because RNA represents the direct output of the genetic information encoded by genomes and a significant proportion of a cell's regulatory capabilities are focused on its synthesis, processing, transport, modification and translation, the generation of such a catalogue is crucial for understanding genome function. Here we report evidence that three-quarters of the human genome is capable of being transcribed, as well as observations about the range and levels of expression, localization, processing fates, regulatory regions and modifications of almost all currently annotated and thousands of previously unannotated RNAs. These observations, taken together, prompt a redefinition of the concept of a gene.

引用

页码：101 / 108

页数：8

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