Evaluation of Precise Optimal Cyclic Strain for Tenogenic Differentiation of MSCs

被引：4
作者：
Morita, Yasuyuki [1 ]
Sato, Toshihiro [1 ]
Watanabe, Sachi [1 ]
Ju, Yang [1 ]
机构：
[1] Nagoya Univ, Grad Sch Engn, Dept Mech Sci & Engn, Chikusa Ku, Furo Cho, Nagoya, Aichi 4648603, Japan
来源：
MECHANICS OF BIOLOGICAL SYSTEMS AND MATERIALS, VOL 6 | 2017年
关键词：
Digital image correlation (DIC); Optimal strain; Mechanical stimulation; Tenogenic differentiation; Mesenchymal stem cell (MSC); MECHANICAL STRETCH; TENDON REPAIR; STEM; EXPRESSION; GENE; COLLAGEN; CELLS;
D O I：
10.1007/978-3-319-41351-8_21
中图分类号：
O3 [力学];
学科分类号：
08 ; 0801 ;
摘要：
Although there are a number of papers relating to tenogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) using uniaxial cyclic stretching stimulation with homogeneous strain field, it has been pretty hard to figure out the optimal normal strain in the stretch direction for the differentiation. In the present study, our group has developed a non-uniform strain field system to elucidate the optimal normal strain in one-time experiment in principle. A relationship between the normal strain of membrane and expression levels of the differentiation marker proteins, type I collagen (Col I) and tenascin-C (Tnc), derived from stretched cells was obtained. Finally, the rigorous optimal normal strains were clarified 7.9 and 8.5 % for Col I and Tnc, respectively. Additionally, we found that a dependence of protein expression levels with the normal strain of membrane was different in each protein, which would be crucial in the field of embryology and regenerative medicine.
引用
收藏
页码：149 / 155
页数：7
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