Quantum dots-based fluorescent probes for turn-on and turn-off sensing of butyrylcholinesterase

被引：52

作者：

Chen, Zhenzhen ^{[1
]}

Ren, Xiangling ^{[1
,2
]}

Meng, Xianwei ^{[1
]}

Tan, Longfei ^{[1
]}

Chen, Dong ^{[1
]}

Tang, Fangqiong ^{[1
]}

机构：

[1] Chinese Acad Sci, Tech Inst Phys & Chem, Lab Controllable Preparat & Applicat Nanomat, Beijing 100190, Peoples R China

[2] Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2013年 / 44卷

基金：

中国国家自然科学基金;

关键词：

Quantum dots; Butyrylcholinesterase; Fluorescence; Detection; Turn-on; Turn-off; RESONANCE ENERGY-TRANSFER; OPTICAL-DETECTION; GLUCOSE; NANOPARTICLES; INHIBITION; MECHANISM; EXPOSURE; COMPLEX; CHOLINE; GROWTH;

D O I：

10.1016/j.bios.2013.01.034

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A novel turn-on and turn-off sensor based on the fluorescence change of quantum dots (QDs) has been developed to detect the activity of butyrylcholinesterase (BChE). In the turn-on sensing system, we realized the detection of BChE with just one enzyme. A linear calibration plot of the activity of BChE was obtained in the wide amounts range from 10 to 1000 U/L and the detection limit was 10 U/L. In the turn-off sensing system, we realized the sensing of BChE with a wide linear relationship of 10-2000 U/L which was much wider than many other detection methods. We also studied the application in serum sample detection. The BChE sensor shows great performances. Furthermore, the turn-on and turn-off sensing mechanisms were studied extensively. These results showed that our strategy would most probably be applicable in assembling diagnostic micro-device for realizing the rapid clinical analysis of BChE. (c) 2013 Elsevier B.V. All rights reserved.

引用

页码：204 / 209

页数：6

共 27 条

[1] Electrochemistry and electrogenerated chemiluminescence of CdTe nanoparticles [J].