Development of an extraction method for the determination of prostaglandins in biological tissue samples using liquid chromatography-tandem mass spectrometry: Application to gonads of Atlantic cod (Gadus morhua)

被引：2

作者：

Janagap, Steve ^{[1
,2
]}

Azeredo, Rita ^{[1
]}

Thu-Thao Nguyen ^{[1
]}

Hamre, Kristin ^{[1
]}

Araujo, Pedro ^{[1
]}

机构：

[1] Natl Inst Nutr & Seafood Res NIFES, N-5817 Bergen, Norway

[2] Univ Philippines Visayas, Coll Arts & Sci, Dept Chem, Miagao 5023, Iloilo, Philippines

来源：

ANALYTICA CHIMICA ACTA | 2012年 / 749卷

关键词：

Gonads; Prostaglandins; Prostaglandin E-2; Prostaglandin F-2a; Liquid chromatography-tandem mass; spectrometry; Atlantic cod; TROUT SALVELINUS-FONTINALIS; PERCH PERCA-FLAVESCENS; PATINOPECTEN-YESSOENSIS; INDUCED OVULATION; INDOMETHACIN; OVARIAN; COMPONENTS; FOLLICLE; GOLDFISH; SCALLOP;

D O I：

10.1016/j.aca.2012.08.039

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple extraction method for the analysis of PGE(2) and PGF(2a) in gonad samples from Atlantic cod and further quantification by using liquid chromatography-tandem mass spectrometry is proposed. The evaluation of the best solvent extraction conditions and the analytical performance parameters are reported. The method was highly selective for both prostaglandins and the calibration curves, based on the internal standard method, were linear between 5 and 1000 ng mL(-1) for PGE(2) and PGF(2a) with limits of detection of 1 ng mL(-1) and 1.5 ng mL(-1) and recovery values of 99.999 +/- 0.002 and 99.967 +/- 0.023 respectively. The homogenization of samples using liquid nitrogen combined with the developed extraction protocol can be implemented in different types of biological tissues. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：51 / 55

页数：5

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