DNA barcoding of arid wild plants using rbcL gene sequences

被引：42
作者：
Bafeel, S. O. [2 ]
Arif, I. A. [1 ,3 ]
Bakir, M. A. [1 ,3 ]
Al Homaidan, A. A. [1 ,3 ]
Al Farhan, A. H. [1 ,3 ]
Khan, H. A. [1 ,3 ]
机构：
[1] King Saud Univ, Coll Sci, Prince Sultan Res Chair Environm & Wildlife, Riyadh 11451, Saudi Arabia
[2] King Abdulaziz Univ, Dept Biol, Coll Sci, Jeddah 21413, Saudi Arabia
[3] King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia
来源：
GENETICS AND MOLECULAR RESEARCH | 2012年 / 11卷 / 03期
关键词：
DNA barcoding; rbcL; Wild plants; Identification; Phylogenetics; MATK; DIVERSITY;
D O I：
10.4238/2012.July.19.12
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA barcoding is currently gaining popularity due to its simplicity and high accuracy as compared to the complexity and subjective biases associated with morphology-based identification of taxa. The standard chloroplast DNA barcode for land plants recommended by the Consortium for the Barcode of Life (CBOL) plant working group needs to be evaluated for a wide range of plant species. We therefore tested the potential of the rbcL marker for the identification of wild plants belonging to diverse families of arid regions. Maximum likelihood tree analysis was performed to evaluate the discriminatory power of the rbcL gene. Our findings showed that using rbcL gene sequences enabled identification of the majority of the samples (92%) to genus level and only 17% to species level.
引用
收藏
页码：1934 / 1941
页数：8
相关论文
共 33 条
  • [11] Conservation of selection on matK following an ancient loss of its flanking intron
    Duffy, Aaron M.
    Kelchner, Scot A.
    Wolf, Paul G.
    [J]. GENE, 2009, 438 (1-2) : 17 - 25
  • [12] Multiple Multilocus DNA Barcodes from the Plastid Genome Discriminate Plant Species Equally Well
    Fazekas, Aron J.
    Burgess, Kevin S.
    Kesanakurti, Prasad R.
    Graham, Sean W.
    Newmaster, Steven G.
    Husband, Brian C.
    Percy, Diana M.
    Hajibabaei, Mehrdad
    Barrett, Spencer C. H.
    [J]. PLOS ONE, 2008, 3 (07):
  • [13] FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x
  • [14] Graham S, 2002, GLOBAL ESTIMATE ENDA
  • [15] The promise of DNA barcoding for taxonomy
    Hebert, PDN
    Gregory, TR
    [J]. SYSTEMATIC BIOLOGY, 2005, 54 (05) : 852 - 859
  • [16] Hegazy A.K., 2009, Acta Ecol. Sin, V29, P20, DOI [10.1016/j.chnaes.2009.04.004, DOI 10.1016/J.CHNAES.2009.04.004, DOI 10.1626/JCS.36.4354]
  • [17] Selecting barcoding loci for plants: evaluation of seven candidate loci with species-level sampling in three divergent groups of land plants
    Hollingsworth, Michelle L.
    Clark, Alex Andra
    Forrest, Laura L.
    Richardson, James
    Pennington, R. Toby
    Long, David G.
    Cowan, Robyn
    Chase, Mark W.
    Gaudeul, Myriam
    Hollingsworth, Peter M.
    [J]. MOLECULAR ECOLOGY RESOURCES, 2009, 9 (02) : 439 - 457
  • [18] Joshi K, 2004, CURR SCI INDIA, V87, P159
  • [19] Spatial patterns of plant diversity below-ground as revealed by DNA barcoding
    Kesanakurti, Prasad R.
    Fazekas, Aron J.
    Burgess, Kevin S.
    Percy, Diana M.
    Newmaster, Steven G.
    Graham, Sean W.
    Barrett, Spencer C. H.
    Hajibabaei, Mehrdad
    Husband, Brian C.
    [J]. MOLECULAR ECOLOGY, 2011, 20 (06) : 1289 - 1302
  • [20] Use of DNA barcodes to identify flowering plants
    Kress, WJ
    Wurdack, KJ
    Zimmer, EA
    Weigt, LA
    Janzen, DH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (23) : 8369 - 8374
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