DNA barcoding of arid wild plants using rbcL gene sequences

被引：42

作者：

Bafeel, S. O. ^{[2
]}

Arif, I. A. ^{[1
,3
]}

Bakir, M. A. ^{[1
,3
]}

Al Homaidan, A. A. ^{[1
,3
]}

Al Farhan, A. H. ^{[1
,3
]}

Khan, H. A. ^{[1
,3
]}

机构：

[1] King Saud Univ, Coll Sci, Prince Sultan Res Chair Environm & Wildlife, Riyadh 11451, Saudi Arabia

[2] King Abdulaziz Univ, Dept Biol, Coll Sci, Jeddah 21413, Saudi Arabia

[3] King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia

来源：

GENETICS AND MOLECULAR RESEARCH | 2012年 / 11卷 / 03期

关键词：

DNA barcoding; rbcL; Wild plants; Identification; Phylogenetics; MATK; DIVERSITY;

D O I：

10.4238/2012.July.19.12

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA barcoding is currently gaining popularity due to its simplicity and high accuracy as compared to the complexity and subjective biases associated with morphology-based identification of taxa. The standard chloroplast DNA barcode for land plants recommended by the Consortium for the Barcode of Life (CBOL) plant working group needs to be evaluated for a wide range of plant species. We therefore tested the potential of the rbcL marker for the identification of wild plants belonging to diverse families of arid regions. Maximum likelihood tree analysis was performed to evaluate the discriminatory power of the rbcL gene. Our findings showed that using rbcL gene sequences enabled identification of the majority of the samples (92%) to genus level and only 17% to species level.

引用

页码：1934 / 1941

页数：8

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