Effects of different promoters on the protective efficacy of recombinant Marek's disease virus type 1 expressing the VP2 gene of infectious bursal disease virus

被引：12

作者：

Li, Kai ^{[1
]}

Liu, Yongzhen ^{[1
]}

Liu, Changjun ^{[1
]}

Gao, Li ^{[1
]}

Zhang, Yanping ^{[1
]}

Gao, Yulong ^{[1
]}

Cui, Hongyu ^{[1
]}

Qi, Xiaole ^{[1
]}

Zhong, Li ^{[1
]}

Wang, Xiaomei ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Avian Immunosuppress Dis Div, Harbin 150069, Peoples R China

来源：

VACCINE | 2016年 / 34卷 / 47期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

Recombinant MDV1; Infectious bursal disease virus; Promoters; VP2; protein; Protective efficacy; AVIAN INFLUENZA-VIRUS; TRANSCRIPTIONAL ACTIVITY; MATERNAL ANTIBODIES; HEMAGGLUTININ GENE; INDUCE PROTECTION; NEWCASTLE-DISEASE; IBDV VP2; CHICKENS; HERPESVIRUS; VACCINE;

D O I：

10.1016/j.vaccine.2016.10.008

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The vaccine efficacy of recombinant viruses can be influenced by many factors. Accordingly, the activity of promoters has been one of the major factors affecting the antigen expression and protection rate. In the present study, two recombinant Marek's disease virus type 1 (MDV1) vaccines containing the VP2 gene of infectious bursal disease virus (IBDV) under control of different promoters were generated from overlapping fosmid DNAs. The rMDV-Pec-VP2 virus containing the VP2 gene under control of the Pec promoter (CMV enhancer and chicken beta-actin chimera promoter) demonstrated higher VP2 expression and stronger antibody response against IBDV in chickens than the rMDV-CMV-VP2 virus using the CMV promoter. After IBDV lethal challenge in specific-pathogen-free chickens, rMDV-Pec-VP2 provided complete protection against developing mortality, clinical signs, and the formation of bursal lesions, which was better than that provided by rMDV-CMV-VP2. Our findings indicate that the protective efficacy of the recombinant MDV1 vaccine against IBDV highly correlates with VP2 expression. This recombinant MDV1 vaccine expressing VP2 could have significant potential as a bivalent vaccine against both virulent IBDV and MDV infections in chickens. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：5744 / 5750

页数：7

共 33 条

[1] OUTBREAK OF VIRULENT INFECTIOUS BURSAL DISEASE IN EAST ANGLIA [J].