Surfactant free synthesis of cationic nano-vesicles: A safe triple drug loaded vehicle for the topical treatment of cutaneous leishmaniasis

被引：14

作者：

Khalid, Sidra ^{[1
,2
]}

Salman, Saad ^{[3
]}

Iqbal, Kashif ^{[4
]}

Rehman, Fiza Ur ^{[1
]}

Ullah, Iffat ^{[4
]}

Satoskar, Abhay R. ^{[5
]}

Khan, Gul Majid ^{[1
,6
]}

Dar, M. Junaid ^{[4
]}

机构：

[1] Quaid I Azam Univ, Fac Biol Sci, Dept Pharm, Islamabad, Pakistan

[2] Drug Regulatory Author Pakistan DRAP, Div Pharmaceut Evaluat & Registrat, Islamabad, Pakistan

[3] Capital Univ Sci & Technol, Fac Pharm, Islamabad, Pakistan

[4] Univ Lahore, Sch Pharm, Nanomed Res Grp, Islamabad Campus, Islamabad, Pakistan

[5] Ohio State Univ, Dept Pathol, Med Ctr, Columbus, OH USA

[6] Islamia Coll Univ, Peshawar, Khyber Pakhtunk, Pakistan

来源：

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE | 2022年 / 40卷

关键词：

Leishmaniasis; Cationic transfersomes; Macrophage targeting; Surfactant free nano-vesicles; IN-VITRO; MEGLUMINE ANTIMONIATE; DELIVERY-SYSTEMS; NANOPARTICLES; MILTEFOSINE; IMIQUIMOD; LIPOSOMES;

D O I：

10.1016/j.nano.2021.102490

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The basic aim of the study was to develop and evaluate the triple drug loaded cationic nano-vesicles (cNVs), where miltefosine was used as a replacement of surfactant (apart from its anti-leishmanial role), in addition to meglumine antimoniate (MAM) and imiquimod (Imq), as a combination therapy for the topical treatment of cutaneous leishmaniasis (CL). The optimized formulation was nano-sized (86.2 +/- 2.7 nm) with high entrapment efficiency (63.8 +/- 2.1% (MAM) and 81.4 +/- 2.3% (Imq)). In-vivo skin irritation assay showed reduced irritation potential and a decrease in the cytotoxicity of cNVs as compared to conventional NVs (having sodium deoxycholate as a surfactant). A synergistic interaction between drugs was observed against intracellular amastigotes, whereas the in-vivo antileishmanial study presented a significant reduction in the parasitic burden. The results suggested the potential of surfactant free, triple drug loaded cNVs as an efficient vehicle for the safe topical treatment of CL. (c) 2021 Elsevier Inc. All rights reserved.

引用

页数：10

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