Vectorization and Intracellular Distribution of a Two-Photon-Absorbing, Near-Infrared-Emitting π-Extended Boranil Dye

被引：29

作者：

Frath, Denis ^{[1
]}

Didier, Pascal ^{[1
]}

Mely, Yves ^{[1
]}

Massue, Julien ^{[1
]}

Ulrich, Gilles ^{[1
]}

机构：

[1] UMR CNRS 7515, F-67087 Strasbourg 02, France

来源：

CHEMPHOTOCHEM | 2017年 / 1卷 / 04期

关键词：

boranil dyes; fluorescence lifetime imaging microscopy; fluorophores; imaging agents; two-photon absorption; 2-PHOTON ABSORPTION; FLUORESCENCE; CHROMOPHORES;

D O I：

10.1002/cptc.201700012

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A p-extended boranil (PEB) dye has been synthesized and its photophysical properties have been recorded in a range of solvents. The dye exhibits an intense solvatochromic fluorescence emission covering the entire visible spectrum and the near infra-red (NIR) region (from lambda=534 to 764 nm) with quantum yields up to 69 %. Embedment of the PEB dye in the amphiphilic block copolymer Cremophor EL (R) leads to a strong emission (lambda(em)=617 nm, Phi=0.33) in PBS buffer. Moreover, twophoton absorption (TPA) spectroscopy evidenced a large twophoton (2P) cross section (420 GM), enabling the use of the PEB dye in 2P-excitation fluorescence microscopy. The cellular uptake and distribution of PEB in HeLa cells was monitored using fluorescence lifetime imaging microscopy (FLIM) and real-time widefield imaging. It was found that the emission of the dye is strongly influenced by the local environment and that the dye is preferentially internalized in acidic vesicles.

引用

页码：109 / 112

页数：4

共 37 条

[1] POLY(ETHYLENE OXIDE)-POLY(PROPYLENE OXIDE)-POLY(ETHYLENE OXIDE) BLOCK-COPOLYMER SURFACTANTS IN AQUEOUS-SOLUTIONS AND AT INTERFACES - THERMODYNAMICS, STRUCTURE, DYNAMICS, AND MODELING [J].