Molecular resolution imaging by repetitive optical selective exposure

被引：128

作者：

Gu, Lusheng ^{[1
,2
,3
,4
]}

Li, Yuanyuan ^{[1
,2
]}

Zhang, Shuwen ^{[1
,5
]}

Xue, Yanhong ^{[1
,2
,4
]}

Li, Weixing ^{[1
,2
,3
]}

Li, Dong ^{[1
,4
]}

Xu, Tao ^{[1
,2
,3
,4
,5
]}

Ji, Wei ^{[1
,2
,3
,4
]}

机构：

[1] Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing, Peoples R China

[2] Huairou Natl Comprehens Sci Ctr, Phys Sci Lab, Beijing, Peoples R China

[3] Chinese Acad Sci, Inst Biophys, Core Facil Prot Res, Ctr Biol Instrument Dev, Beijing, Peoples R China

[4] Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China

[5] Univ Chinese Acad Sci, Sch Future Technol, Beijing, Peoples R China

来源：

NATURE METHODS | 2019年 / 16卷 / 11期

关键词：

LOCALIZATION; MICROSCOPY; LIMIT;

D O I：

10.1038/s41592-019-0544-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We introduce an interferometric single-molecule localization method for super-resolution fluorescence microscopy. Fluorescence molecules are located by the intensities of multiple excitation patterns of an interference fringe, providing around a twofold improvement in the localization precision compared with the conventional imaging with the same photon budget. We demonstrate this technique by resolving nanostructures down to 5 nm in size over a large 25 x 25 mu m(2) field of view.

引用

页码：1114 / +

页数：7

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