Carboxymethylation modification, characterization, antioxidant activity and anti-UVC ability of Sargassum fusiforme polysaccharide

被引：28

作者：

An, Yongzhen ^{[1
]}

Liu, Haitang ^{[1
,2
]}

Li, Xuexiu ^{[1
]}

Liu, Jing ^{[1
]}

Chen, Lin ^{[1
]}

Jin, Xin ^{[1
]}

Chen, Ting ^{[1
,3
]}

Wang, Wenqian ^{[4
]}

Liu, Zhong ^{[1
]}

Zhang, Meiyun ^{[3
]}

Liu, Fufeng ^{[4
]}

机构：

[1] Tianjin Univ Sci & Technol, Coll Light Ind Sci & Engn, China Light Ind Key Lab Papermaking & Biorefinery, Tianjin 300457, Peoples R China

[2] Tianjin Univ Sci & Technol, Key Lab Food Nutr & Safety, Tianjin Key Lab Food Nutr & Safety, Minist Educ, Tianjin 300457, Peoples R China

[3] Shaanxi Univ Sci & Technol, Coll Bioresources Chem & Mat Engn, Xian 710021, Peoples R China

[4] Tianjin Univ Sci & Technol, Sch Biol Engn, Tianjin, Peoples R China

来源：

CARBOHYDRATE RESEARCH | 2022年 / 515卷

基金：

中国国家自然科学基金;

关键词：

Sargassum fusiforme Polysaccharide; Carboxymethylation; Characterization; Biological activity; IN-VITRO ANTIOXIDANT; SULFATED POLYSACCHARIDES; STRUCTURAL ELUCIDATION; NMR-SPECTROSCOPY; DEGRADATION;

D O I：

10.1016/j.carres.2022.108555

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Taking the degree of substitution (DS) as the index, the carboxymethylation conditions of Sargassum fusiforme polysaccharide (SFP) were studied. According to the single factor experiment results, the optimum experimental conditions were obtained: sodium hydroxide concentration, 15% (20 mL); alkalization temperature, 50 degrees C; dosage of chloroacetic acid 1.5 g; etherification time, 2 h, and the Carboxymethyl Sargassum fusiforme polysaccharide (CSFP) with the highest DS (0.635) was obtained. And then, the physicochemical properties, structural information and bioactivity of SFP and CSFP were characterized. The SFP and CSFP were composed of four monosaccharides, with a small amount of protein, and their molecular weights to 780.2 kDa and 386.3 kDa respectively. The results of FTIR and NMR showed that the carboxymethyl was successfully grafted onto the C-4 and C-6 of sugar chain. The results of anti UVC experiment showed that SFP and CSFP had a certain negative effect on cell activity, and the degree of damage caused by UVC radiation was weakened, and the anti UVC performance of CSFP was better than that of SFP.

引用

页数：10

共 58 条

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