Light triggered nanoscale biolistics for efficient intracellular delivery of functional macromolecules in mammalian cells

被引：19

作者：

Fraire, Juan C. ^{[1
]}

Shaabani, Elnaz ^{[1
]}

Sharifiaghdam, Maryam ^{[1
]}

Rombaut, Matthias ^{[2
]}

Hinnekens, Charlotte ^{[1
]}

Hua, Dawei ^{[1
,3
]}

Ramon, Jana ^{[1
]}

Raes, Laurens ^{[1
]}

Bolea-Fernandez, Eduardo ^{[4
]}

Brans, Toon ^{[1
]}

Vanhaecke, Frank ^{[4
]}

Borghgraef, Peter ^{[5
]}

Huang, Chaobo ^{[3
]}

Sauvage, Felix ^{[1
]}

Vanhaecke, Tamara ^{[2
]}

De Kock, Joery ^{[2
]}

Xiong, Ranhua ^{[1
,3
]}

De Smedt, Stefaan ^{[1
,3
]}

Braeckmans, Kevin ^{[1
]}

机构：

[1] Univ Ghent, Fac Pharmaceut Sci, Lab Gen Biochem & Phys Pharm, B-9000 Ghent, Belgium

[2] Vrije Univ Brussel VUB, Fac Med & Pharm, Dept In Vitro Toxicol & Dermatocosmetol, B-1090 Brussels, Belgium

[3] Nanjing Forestry Univ NFU, Coll Chem Engn, Joint Lab Adv Biomed Mat NFU UGent, Nanjing 210037, Peoples R China

[4] Univ Ghent, Atom & Mass Spectrometry A&MS Res Grp, Dept Chem, Campus Sterre,Krijgslaan 281-S12, B-9000 Ghent, Belgium

[5] VIB, VIB Bioimaging Core Ghent, B-9000 Ghent, Belgium

来源：

NATURE COMMUNICATIONS | 2022年 / 13卷 / 01期

基金：

欧洲研究理事会;

关键词：

GENE-TRANSFER; LASER; NANOPARTICLES; TRANSFECTION; OPTOPORATION; MEMBRANE; PLGA;

D O I：

10.1038/s41467-022-29713-7

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Biolistic intracellular delivery of functional macromolecules makes use of dense microparticles which are ballistically fired onto cells with a pressurized gun. While it has been used to transfect plant cells, its application to mammalian cells has met with limited success mainly due to high toxicity. Here we present a more refined nanotechnological approach to biolistic delivery with light-triggered self-assembled nanobombs (NBs) that consist of a photothermal core particle surrounded by smaller nanoprojectiles. Upon irradiation with pulsed laser light, fast heating of the core particle results in vapor bubble formation, which propels the nanoprojectiles through the cell membrane of nearby cells. We show successful transfection of both adherent and non-adherent cells with mRNA and pDNA, outperforming electroporation as the most used physical transfection technology by a factor of 5.5-7.6 in transfection yield. With a throughput of 10(4)-10(5) cells per second, biolistic delivery with NBs offers scalable and highly efficient transfections of mammalian cells.

引用

页数：16

共 50 条

[11] Cell engineering with microfluidic squeezing preserves functionality of primary immune cells in vivo (Publication with Expression of Concern) [J].

DiTommaso, Tia ;

Cole, Julie M. ;

Cassereau, Luke ;

Bugge, Joshua A. ;

Hanson, Jacquelyn L. Sikora ;

Bridgen, Devin T. ;

Stokes, Brittany D. ;

Loughhead, Scott M. ;

Beutel, Bruce A. ;

Gilbert, Jonathan B. ;

Nussbaum, Kathrin ;

Sorrentino, Antonio ;

Toggweiler, Janine ;

Schmidt, Tobias ;

Gyuelveszi, Gabor ;

Bernstein, Howard ;

Sharei, Armon .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2018, 115 (46) :E10907-E10914

[12] Uptake, distribution, clearance, and toxicity of iron oxide nanoparticles with different sizes and coatings [J].

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Huang, Jian ;

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Xiao, Kai .

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[13] GENE-TRANSFER TO TUMOR-INFILTRATING LYMPHOCYTES AND OTHER MAMMALIAN SOMATIC-CELLS BY MICROPROJECTILE BOMBARDMENT [J].

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[14] Vapor nanobubble is the more reliable photothermal mechanism for inducing endosomal escape of siRNA without disturbing cell homeostasis [J].

Fraire, Juan C. ;

Houthaeve, Gaelle ;

Liu, Jing ;

Raes, Laurens ;

Vermeulen, Lotte ;

Stremersch, Stephan ;

Brans, Toon ;

Barriga, Gerardo Garcia-Diaz ;

De Keulenaer, Sarah ;

Van Nieuwerburgh, Filip ;

De Rycke, Riet ;

Vandesompele, Jo ;

Mestdagh, Pieter ;

Raemdonck, Koen ;

De Vos, Winnok H. ;

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Braeckmans, Kevin .

JOURNAL OF CONTROLLED RELEASE, 2020, 319 :262-275

[15] Nanoparticle-sensitized photoporation enables inflammasome activation studies in targeted single cells† [J].

Harizaj, Aranit ;

Van Hauwermeiren, Filip ;

Stremersch, Stephan ;

De Rycke, Riet ;

De Keersmaecker, Herlinde ;

Brans, Toon ;

Fraire, Juan C. ;

Grauwen, Karolien ;

De Smedt, Stefaan C. ;

Lentacker, Ine ;

Lamkanfi, Mohamed ;

Braeckmans, Kevin .

NANOSCALE, 2021, 13 (13) :6592-6604

[16] Gold Nanoparticle Mediated Laser Transfection for Efficient siRNA Mediated Gene Knock Down [J].

Heinemann, Dag ;

Schomaker, Markus ;

Kalies, Stefan ;

Schieck, Maximilian ;

Carlson, Regina ;

Escobar, Hugo Murua ;

Ripken, Tammo ;

Meyer, Heiko ;

Heisterkamp, Alexander .

PLOS ONE, 2013, 8 (03)

[17] MEMBRANE PERFORATION AND RECOVERY DYNAMICS IN MICROBUBBLE-MEDIATED SONOPORATION [J].

Hu, Yaxin ;

Wan, Jennifer M. F. ;

Yu, Alfred C. H. .

ULTRASOUND IN MEDICINE AND BIOLOGY, 2013, 39 (12) :2393-2405

[18] An easy-to-prepare microshotgun for efficient transmembrane delivery by powering nanoparticles [J].

Liang, Zhongping ;

Yu, Hang ;

Lai, Jianfeng ;

Wen, Lu ;

Chen, Gang .

JOURNAL OF CONTROLLED RELEASE, 2020, 321 :119-131

[19] Laser Pulse Duration Is Critical For the Generation of Plasmonic Nanobubbles [J].

Lukianova-Hleb, Ekaterina Y. ;

Volkov, Alexey N. ;

Lapotko, Dmitri O. .

LANGMUIR, 2014, 30 (25) :7425-7434

[20] Poly Lactic-co-Glycolic Acid (PLGA) as Biodegradable Controlled Drug Delivery Carrier [J].

Makadia, Hirenkumar K. ;

Siegel, Steven J. .

POLYMERS, 2011, 3 (03) :1377-1397

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