The heterogeneity of human CD127+ innate lymphoid cells revealed by single-cell RNA sequencing

被引:393
作者
Bjorklund, Asa K. [1 ,2 ,3 ]
Forkel, Marianne [4 ]
Picelli, Simone [1 ]
Konya, Viktoria [4 ]
Theorell, Jakob [4 ]
Friberg, Danielle [5 ,6 ]
Sandberg, Rickard [1 ,2 ]
Mjosberg, Jenny [4 ]
机构
[1] Ludwig Inst Canc Res, S-10401 Stockholm, Sweden
[2] Karolinska Inst, Dept Cell & Mol Biol, Stockholm, Sweden
[3] Uppsala Univ, Dept Cell & Mol Biol, Sci Life Lab, Uppsala, Sweden
[4] Karolinska Inst, Dept Med Huddinge, Ctr Infect Med, Stockholm, Sweden
[5] Karolinska Univ Hosp, Dept Otorhinolaryngol, Stockholm, Sweden
[6] Karolinska Inst, CLINTEC, Stockholm, Sweden
基金
瑞典研究理事会;
关键词
T-CELL; GENE-EXPRESSION; NK CELLS; SEQ; NOTCH; INDUCER; FETAL; DIFFERENTIATION; TRANSCRIPTOME; PACKAGE;
D O I
10.1038/ni.3368
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Innate lymphoid cells (ILCs) are increasingly appreciated as important participants in homeostasis and inflammation. Substantial plasticity and heterogeneity among ILC populations have been reported. Here we have delineated the heterogeneity of human ILCs through single-cell RNA sequencing of several hundreds of individual tonsil CD127(+) ILCs and natural killer (NK) cells. Unbiased transcriptional clustering revealed four distinct populations, corresponding to ILC1 cells, ILC2 cells, ILC3 cells and NK cells, with their respective transcriptomes recapitulating known as well as unknown transcriptional profiles. The single-cell resolution additionally divulged three transcriptionally and functionally diverse subpopulations of ILC3 cells. Our systematic comparison of single-cell transcriptional variation within and between ILC populations provides new insight into ILC biology during homeostasis, with additional implications for dysregulation of the immune system.
引用
收藏
页码:451 / +
页数:13
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