A bacterium-like particle vaccine displaying Zika virus prM-E induces systemic immune responses in mice

被引:12
|
作者
Jin, Hongli [1 ,2 ]
Bai, Yujie [1 ]
Wang, Jianzhong [3 ]
Jiao, Cuicui [1 ]
Liu, Di [1 ,3 ]
Zhang, Mengyao [1 ]
Li, Entao [2 ]
Huang, Pei [1 ]
Gong, Zhiyuan [1 ]
Song, Yumeng [1 ]
Xu, Shengnan [2 ]
Feng, Na [2 ]
Zhao, Yongkun [2 ]
Wang, Tiecheng [2 ]
Li, Nan [2 ]
Gao, Yuwei [2 ]
Yang, Songtao [2 ]
Zhang, Haili [1 ]
Li, Yuanyuan [1 ]
Xia, Xianzhu [1 ,2 ]
Wang, Hualei [1 ]
机构
[1] Jilin Univ, Coll Vet Med, Key Lab Zoonosis Res, Minist Educ, Changchun, Peoples R China
[2] Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China
[3] Jilin Agr Univ, Coll Vet Med, Changchun, Peoples R China
关键词
bacterium-like particle; GEM; prM-E protein; ZIKV; LACTOCOCCUS-LACTIS; DENDRITIC CELLS; DNA VACCINE; PROTEINS; ANTIBODY; EXPRESSION; COMPLEX; SURFACE;
D O I
10.1111/tbed.14594
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The emergence of Zika virus (ZIKV) infection, which is unexpectedly associated with congenital defects, has prompted the development of safe and effective vaccines. The Gram-positive enhancer matrix-protein anchor (GEM-PA) display system has emerged as a versatile and highly effective platform for delivering target proteins in vaccines. In this study, we developed a bacterium-like particle vaccine, ZI-Delta-PA-GEM, based on the GEM-PA system. The fusion protein ZI-Delta-PA, which contains the prM-E-Delta TM protein of ZIKV (with a stem-transmembrane region deletion) and the protein anchor PA3, was expressed. The fusion protein was successfully displayed on the GEM surface to form ZI-Delta-PA-GEM. Moreover, the intramuscular immunization of BALB/c mice with ZI-Delta-PA-GEM combined with ISA 201 VG and poly(I:C) adjuvants induced durable ZIKV-specific IgG and protective neutralizing antibody responses. Potent B-cell/DC activation was also stimulated early after immunization. Notable, splenocyte proliferation, the secretion of multiple cytokines, T/B-cell activation and central memory T-cell responses were elicited. These data indicate that ZI-Delta-PA-GEM is a promising bacterium-like particle vaccine candidate for ZIKV.
引用
收藏
页码:E2516 / E2529
页数:14
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