Should we ignore western blots when selecting antibodies for other applications?

被引:8
作者
Lund-Johansen, Fridtjof [1 ,2 ]
Browning, Michael D. [3 ]
机构
[1] Oslo Univ Hosp, KG Jebsen Ctr Canc Immunotherapy, Oslo, Norway
[2] Oslo Univ Hosp, Dept Immunol, Oslo, Norway
[3] PhosphoSolutions, Colorado Biosci Pk, Aurora, CO USA
关键词
VALIDATION;
D O I
10.1038/nmeth.4192
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
引用
收藏
页码:215 / 215
页数:1
相关论文
共 6 条
[1]   Anti-Epo receptor antibodies do not predict Epo receptor expression [J].
Elliott, S ;
Busse, L ;
Bass, MB ;
Lu, H ;
Sarosi, I ;
Sinclair, AM ;
Spahr, C ;
Um, M ;
Van, G ;
Begley, CG .
BLOOD, 2006, 107 (05) :1892-1895
[2]   Ten commercial antibodies for alpha-1-adrenergic receptor subtypes are nonspecific [J].
Jensen, Brian C. ;
Swigart, Philip M. ;
Simpson, Paul C. .
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2009, 379 (04) :409-412
[3]   Antibody crossreactivity between the tumour suppressor PHLPP1 and the proto-oncogene β-catenin [J].
Lobert, Viola H. ;
Bruun, Jarle ;
Abrahamsen, Hilde ;
Lothe, Ragnhild A. ;
Stenmark, Harald ;
Kolberg, Matthias ;
Campsteijn, Coen .
EMBO REPORTS, 2013, 14 (01) :10-+
[4]   Commercial Cdk1 antibodies recognize the centrosomal protein Cep152 [J].
Lukinavicius, Grazvydas ;
Lavogina, Darja ;
Goenczy, Pierre ;
Johnsson, Kai .
BIOTECHNIQUES, 2013, 55 (03) :111-+
[5]   Antibody validation by combining immunohistochemistry and protein extraction from formalin-fixed paraffin-embedded tissues [J].
Schuster, Claudia ;
Malinowsky, Katharina ;
Liebmann, Sibylle ;
Berg, Daniela ;
Wolff, Claudia ;
Tran, Kai ;
Schott, Christina ;
Reu, Simone ;
Neumann, Jens ;
Faber, Claudius ;
Hoefler, Heinz ;
Kirchner, Thomas ;
Becker, Karl-Friedrich ;
Hlubek, Falk .
HISTOPATHOLOGY, 2012, 60 (6B) :E37-E50
[6]  
Uhlen M, 2016, NAT METHODS, V13, P823, DOI [10.1038/nmeth.3995, 10.1038/NMETH.3995]