The RNA Atlas expands the catalog of human non-coding RNAs

被引：92

作者：

Lorenzi, Lucia ^{[1
,2
]}

Chiu, Hua-Sheng ^{[3
]}

Avila Cobos, Francisco ^{[1
,2
]}

Gross, Stephen ^{[4
]}

Volders, Pieter-Jan ^{[1
,2
,5
]}

Cannoodt, Robrecht ^{[1
,2
,6
,7
,8
]}

Nuytens, Justine ^{[1
,2
]}

Vanderheyden, Katrien ^{[1
,2
]}

Anckaert, Jasper ^{[1
,2
]}

Lefever, Steve ^{[1
,2
]}

Tay, Aidan P. ^{[9
,10
]}

de Bony, Eric J. ^{[1
,2
]}

Trypsteen, Wim ^{[1
,2
]}

Gysens, Fien ^{[1
,2
]}

Vromman, Marieke ^{[1
,2
]}

Goovaerts, Tine ^{[11
]}

Hansen, Thomas Birkballe ^{[12
]}

Kuersten, Scott ^{[4
]}

Nijs, Nele ^{[13
]}

Taghon, Tom ^{[14
]}

Vermaelen, Karim ^{[15
]}

Bracke, Ken R. ^{[15
]}

Saeys, Yvan ^{[5
,6
]}

De Meyer, Tim ^{[2
,11
]}

Deshpande, Nandan P. ^{[16
]}

Anande, Govardhan ^{[17
,18
]}

Chen, Ting-Wen ^{[19
]}

Wilkins, Marc R. ^{[16
]}

Unnikrishnan, Ashwin ^{[17
,18
]}

De Preter, Katleen ^{[1
,2
]}

Kjems, Jorgen ^{[12
]}

Koster, Jan ^{[20
]}

Schroth, Gary P. ^{[4
]}

Vandesompele, Jo ^{[1
,2
]}

Sumazin, Pavel ^{[2
]}

Mestdagh, Pieter ^{[1
,2
]}

机构：

[1] Univ Ghent, Ctr Med Genet, Ghent, Belgium

[2] Canc Res Inst Ghent CRIG, Ghent, Belgium

[3] Baylor Coll Med, Texas Childrens Canc Ctr, Houston, TX 77030 USA

[4] Illumina Inc, San Diego, CA USA

[5] Univ Ghent VIB, Ctr Med Biotechnol, Ghent, Belgium

[6] VIB, Data Min & Modelling Biomed Grp, Ctr Inflammat Res, Ghent, Belgium

[7] Univ Ghent, Dept Appl Math Comp Sci & Stat, Ghent, Belgium

[8] Data Intuit, Lebbeke, Belgium

[9] CSIRO, Australian E Hlth Res Ctr, Sydney, NSW, Australia

[10] Macquarie Univ, Dept Biomed Sci, Sydney, NSW, Australia

[11] Univ Ghent, Dept Data Anal & Math Modelling, Ghent, Belgium

[12] Aarhus Univ, Dept Mol Biol & Genet, Interdisciplinary Nanosci Ctr iNANO, Aarhus, Denmark

[13] Biogazelle, Zwijnaarde, Belgium

[14] Univ Ghent, Dept Diagnost Sci, Ghent, Belgium

[15] Univ Ghent, Dept Resp Med, Ghent, Belgium

[16] UNSW Sydney, Sch Biotechnol & Biomol Sci, Syst Biol Initiat, Sydney, NSW, Australia

[17] UNSW Sydney, Adult Canc Program, Lowy Canc Res Ctr, Sydney, NSW, Australia

[18] UNSW Sydney, Prince Wales Clin Sch, Sydney, NSW, Australia

[19] Natl Yang Ming Chiao Tung Univ, Inst Bioinformat & Syst Biol, Hsinchu, Taiwan

[20] Univ Amsterdam, Amsterdam UMC, Dept Oncogen, Amsterdam, Netherlands

来源：

NATURE BIOTECHNOLOGY | 2021年 / 39卷 / 11期

基金：

欧盟地平线“2020”; 英国医学研究理事会;

关键词：

OPEN-ACCESS DATABASE; CIRCULAR RNAS; SEQ DATA; TRANSCRIPTION; LANDSCAPE; CANCER; GENE; RECONSTRUCTION; ANNOTATIONS; PROMOTERS;

D O I：

10.1038/s41587-021-00936-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The human transcriptome from 300 tissues and cell lines reveals novel non-coding RNAs. Existing compendia of non-coding RNA (ncRNA) are incomplete, in part because they are derived almost exclusively from small and polyadenylated RNAs. Here we present a more comprehensive atlas of the human transcriptome, which includes small and polyA RNA as well as total RNA from 300 human tissues and cell lines. We report thousands of previously uncharacterized RNAs, increasing the number of documented ncRNAs by approximately 8%. To infer functional regulation by known and newly characterized ncRNAs, we exploited pre-mRNA abundance estimates from total RNA sequencing, revealing 316 microRNAs and 3,310 long non-coding RNAs with multiple lines of evidence for roles in regulating protein-coding genes and pathways. Our study both refines and expands the current catalog of human ncRNAs and their regulatory interactions. All data, analyses and results are available for download and interrogation in the R2 web portal, serving as a basis for future exploration of RNA biology and function.

引用

页码：1453 / +

页数：23

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