A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells

被引：6

作者：

Xi, Haibin ^{[1
,2
]}

Langerman, Justin ^{[3
]}

Sabri, Shan ^{[3
]}

Chien, Peggie ^{[1
,2
,4
]}

Young, Courtney S. ^{[5
,13
]}

Younesi, Shahab ^{[1
,2
]}

Hicks, Michael ^{[1
,2
]}

Gonzalez, Karen ^{[6
,14
,15
]}

Fujiwara, Wakana ^{[7
,16
]}

Marzi, Julia ^{[8
,9
]}

Liebscher, Simone ^{[8
]}

Spencer, Melissa ^{[2
,4
,5
]}

Van Handel, Ben ^{[10
]}

Evseenko, Denis ^{[10
]}

Schenke-Layland, Katja ^{[8
,9
,11
]}

Plath, Kathrin ^{[2
,3
,4
,12
]}

Pyle, April D. ^{[1
,2
,4
,12
]}

机构：

[1] Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA USA

[2] Univ Calif Los Angeles, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell, Los Angeles, CA USA

[3] Univ Calif Los Angeles, David Geffen Sch Med, Dept Biol Chem, Los Angeles, CA 90095 USA

[4] Univ Calif Los Angeles, Mol Biol Inst, Los Angeles, CA USA

[5] Univ Calif Los Angeles, Dept Neurol, Los Angeles, CA 90024 USA

[6] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA USA

[7] Univ Calif Los Angeles, Dept Biochem, Los Angeles, CA USA

[8] Eberhard Karls Univ Tubingen, Res Inst Womens Hlth, Dept Womens Hlth, Tubingen, Germany

[9] Univ Tubingen, Nat & Med Sci Inst NMI, Reutlingen, Germany

[10] Univ Southern Calif, Dept Orthopaed Surg, Keck Sch Med, Stem Cell Res & Regenerat Med, Los Angeles, CA 90007 USA

[11] Univ Calif Los Angeles, Cardiovasc Res Labs, Dept Med Cardiol, Los Angeles, CA USA

[12] Univ Calif Los Angeles, Jonsson Comprehens Canc Ctr, Los Angeles, CA USA

[13] MyoGene Bio, Los Angeles, CA USA

[14] Stanford Univ, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA

[15] Stanford Univ, Dept Biol, Stanford, CA 94305 USA

[16] Georgetown Univ, Sch Med, Washington, DC USA

来源：

CELL STEM CELL | 2020年 / 27卷 / 01期

关键词：

SATELLITE CELLS; GENE-EXPRESSION; SELF-RENEWAL; IN-VIVO; DIFFERENTIATION; TRANSCRIPTION; EXPANSION; GENERATION; QUIESCENCE; MYOGENESIS;

D O I：

10.1016/j.stem.2020.04.017

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The developmental trajectory of human skeletal myogenesis and the transition between progenitor and stem cell states are unclear. We used single-cell RNA sequencing to profile human skeletal muscle tissues from embryonic, fetal, and postnatal stages. In silico, we identified myogenic as well as other cell types and constructed a "roadmap'' of human skeletal muscle ontogeny across development. In a similar fashion, we also profiled the heterogeneous cell cultures generated from multiple human pluripotent stem cell (hPSC) myogenic differentiation protocols and mapped hPSC-derived myogenic progenitors to an embryonic-to-fetal transition period. We found differentially enriched biological processes and discovered co-regulated gene networks and transcription factors present at distinct myogenic stages. This work serves as a resource for advancing our knowledge of human myogenesis. It also provides a tool for a better understanding of hPSC-derived myogenic progenitors for translational applications in skeletal muscle-based regenerative medicine.

引用

页码：158 / +

页数：29

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