Establishment of induced pluripotent stem cell (iPSC) line from an 84-year old patient with late onset Alzheimer's disease (LOAD)

被引：7

作者：

Tancos, Zsuzsanna ^{[1
,2
]}

Varga, Eszter ^{[1
]}

Kovacs, Eszter ^{[1
]}

Dinnyes, Andras ^{[1
,2
]}

Kobolak, Julianna ^{[1
]}

机构：

[1] BioTalentum Ltd, Godollo, Hungary

[2] Szent Istvan Univ, Mol Anim Biotechnol Lab, Godollo, Hungary

来源：

STEM CELL RESEARCH | 2016年 / 17卷 / 01期

关键词：

DIFFERENTIATION;

D O I：

10.1016/j.scr.2016.05.016

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Peripheral blood mononuclear cells (PBMCs) were collected from a clinically characterised 84-year old male with late onset Alzheimer's disease (LOAD). The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated spontaneously towards the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. Our model might offer a good platform to further study the pathomechanism of sporadic AD, to identify early biomarkers and also for drug testing and gene therapy studies. (C) 2016 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license.

引用

页码：75 / 77

页数：3

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