Circadian rhythms in metabolic organs and the microbiota during acute fasting in mice

被引：6

作者：

Pickel, Lauren ^{[1
]}

Lee, Ju Hee ^{[1
,2
]}

Maughan, Heather ^{[3
]}

Shi, Irisa Qianwen ^{[1
]}

Verma, Navkiran ^{[1
,2
]}

Yeung, Christy ^{[1
,2
]}

Guttman, David ^{[4
]}

Sung, Hoon-Ki ^{[1
,2
]}

机构：

[1] Hosp Sick Children, Translat Med Program, Toronto, ON M5G 1X8, Canada

[2] Univ Toronto, Dept Lab Med & Pathol, Toronto, ON, Canada

[3] Ronin Inst, Montclair, NJ USA

[4] Univ Toronto, Ctr Anal Genome Evolut & Funct, Toronto, ON, Canada

来源：

PHYSIOLOGICAL REPORTS | 2022年 / 10卷 / 14期

基金：

加拿大自然科学与工程研究理事会; 加拿大健康研究院;

关键词：

CLOCK; GUT; LIVER; EXPRESSION; GENE; HOMEOSTASIS; MOUSE; DIET; FAT; PHYSIOLOGY;

D O I：

10.14814/phy2.15393

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

The circadian clock regulates metabolism in anticipation of regular changes in the environment. It is found throughout the body, including in key metabolic organs such as the liver, adipose tissues, and intestine, where the timing of the clock is set largely by nutrient signaling. However, the circadian clocks of these tissues during the fasted state have not been completely characterized. Moreover, the sufficiency of a functioning host clock to produce diurnal rhythms in the composition of the microbiome in fasted animals has not been explored. To this end, mice were fasted 24 h prior to collection of key metabolic tissues and fecal samples for the analysis of circadian clock gene expression and microbiome composition. Rhythm characteristics were determined using CircaCompare software. We identify tissue-specific changes to circadian clock rhythms upon fasting, particularly in the brown adipose tissue, and for the first time demonstrate the rhythmicity of the microbiome in fasted animals.

引用

页数：18