Development, validation, and evaluation of an assay for the detection of wood frogs (Rana sylvatica) in environmental DNA (vol 10, pg 631, 2018)

被引：1

作者：

Spangler, Mark A. ^{[1
,2
]}

Huettmann, Falk ^{[1
]}

Herriott, Ian C. ^{[3
]}

Lopez, J. Andres ^{[2
,4
]}

机构：

[1] UAF, Dept Biol & Wildlife, Inst Arctic Biol, EWHALE Lab, 902 N Koyukuk Dr, Fairbanks, AK 99775 USA

[2] Univ Alaska Museum North UAM, 907 Yukon Dr, Fairbanks, AK 99775 USA

[3] UAF, Inst Arctic Biol, DNA Core Facil, 909 N Koyukuk Dr, Fairbanks, AK 99775 USA

[4] UAF, Coll Fisheries & Ocean Sci, Div Fisheries, 905 N Koyukuk Dr, Fairbanks, AK 99775 USA

来源：

CONSERVATION GENETICS RESOURCES | 2018年 / 10卷 / 04期

关键词：

Alaska; Cytochrome B; eDNA; Rana sylvatica; Wood frog;

D O I：

10.1007/s12686-017-0916-9

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

We developed and describe a qPCR assay for the detection of wood frogs (Rana sylvatica) using environmental DNA (eDNA) sampling. A single primer set was designed to amplify a 115-bp region of the wood frog cytochrome B gene and assessed for target specificity. There was no evidence of amplification in 11 non-target species. We evaluated the utility of the primer set in qPCR assay by conducting geo-referenced eDNA field surveys in Interior Alaska. Results indicate that the assay consistently detects wood frog DNA in the environment to 1.83 x 10(-3) pg/mu L. The assay provides a complement to traditional survey methods and can be readily applied in a wider conservation and management context.

引用

页码：917 / 917

页数：1