Development, validation, and evaluation of an assay for the detection of wood frogs (Rana sylvatica) in environmental DNA

被引：1

作者：

Spangler, Mark A. ^{[1
,2
]}

Huettmann, Falk ^{[1
]}

Herriott, Ian C. ^{[3
]}

Lopez, J. Andres ^{[2
,4
]}

机构：

[1] UAF, EWHALE Lab, Inst Arctic Biol, Dept Biol & Wildlife, 902 N Koyukuk Dr, Fairbanks, AK 99775 USA

[2] Univ Alaska Museum North UAM, 907 Yukon Dr, Fairbanks, AK 99775 USA

[3] UAF, DNA Core Facil, Inst Arctic Biol, 909 N Koyukuk Dr, Fairbanks, AK 99775 USA

[4] UAF, Coll Fisheries & Ocean Sci, Div Fisheries, 905 N Koyukuk Dr, Fairbanks, AK 99775 USA

来源：

CONSERVATION GENETICS RESOURCES | 2018年 / 10卷 / 04期

关键词：

eDNA; Wood frog; Rana sylvatica; Cytochrome B; Alaska; EDNA; SAMPLES;

D O I：

10.1007/s12686-017-0881-3

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

We developed and describe a qPCR assay for the detection of wood frogs (Rana sylvatica) using environmental DNA (eDNA) sampling. A single primer set was designed to amplify a 115-bp region of the wood frog cytochrome B gene and assessed for target specificity. There was no evidence of amplification in 11 non-target species. We evaluated the utility of the primer set in qPCR assay by conducting geo-referenced eDNA field surveys in Interior Alaska. Results indicate that the assay consistently detects wood frog DNA in the environment to 1.83 x 10(-3) pg/mu L. The assay provides a complement to traditional survey methods and can be readily applied in a wider conservation and management context.

引用

页码：631 / 633

页数：3

共 50 条

[1] Development, validation, and evaluation of an assay for the detection of wood frogs (Rana sylvatica) in environmental DNA
Mark A. Spangler
Falk Huettmann
Ian C. Herriott
J. Andrés López
Conservation Genetics Resources, 2018, 10 : 631 - 633
[2] Selective phonotaxis by male wood frogs (Rana sylvatica) to the sound of a chorus
Bee, Mark A.
BEHAVIORAL ECOLOGY AND SOCIOBIOLOGY, 2007, 61 (06) : 955 - 966
[3] The effects of oil sands wetlands on wood frogs (Rana sylvatica)
Hersikorn B.D.
Ciborowski J.J.C.
Smits J.E.G.
Toxicological and Environmental Chemistry, 2010, 92 (08) : 1513 - 1527
[4] Age and size of wood frogs, Rana sylvatica, from Kuujjuarapik, northern Quebec
Leclair, R
Leclair, MH
Dubois, J
Daoust, JL
CANADIAN FIELD-NATURALIST, 2000, 114 (03): : 381 - 387
[5] Pathogenesis of Frog Virus 3 (Ranavirus, Iridoviridae) Infection in Wood Frogs (Rana sylvatica)
Forzan, M. J.
Jones, K. M.
Ariel, E.
Whittington, R. J.
Wood, J.
Markham, R. J. Frederick
Daoust, P. -Y.
VETERINARY PATHOLOGY, 2017, 54 (03) : 531 - 548
[6] Selective phonotaxis by male wood frogs (Rana sylvatica) to the sound of a chorus
Mark A. Bee
Behavioral Ecology and Sociobiology, 2007, 61 : 955 - 966
[7] The effect of habitat fragmentation on finescale population structure of wood frogs (Rana sylvatica)
M. Kathrine A. Crosby
Lawrence E. Licht
Jinzhong Fu
Conservation Genetics, 2009, 10 : 1707 - 1718
[8] Novel tetranucleotide microsatellite DNA markers for the wood frog, Rana sylvatica
Julian, SE
King, TL
MOLECULAR ECOLOGY NOTES, 2003, 3 (02): : 256 - 258
[9] The effect of habitat fragmentation on finescale population structure of wood frogs (Rana sylvatica)
Crosby, M. Kathrine A.
Licht, Lawrence E.
Fu, Jinzhong
CONSERVATION GENETICS, 2009, 10 (06) : 1707 - 1718
[10] Ranavirus in wood frogs (Rana sylvatica):: Potential sources of transmission within and between ponds
Harp, Elizabeth M.
Petranka, James W.
JOURNAL OF WILDLIFE DISEASES, 2006, 42 (02) : 307 - 318

← 1 2 3 4 5 →