Interplay of diverse adjuvants and nanoparticle presentation of native-like HIV-1 envelope trimers (vol 6, 134, 2021)

被引：4
|
作者：
Sliepen, Kwinten
Schermer, Edith
Bontjer, Ilja
Burger, Judith A.
Levai, Reka Felfodine
Mundsperger, Philipp
Brouwer, Philip J. M.
Tolazzi, Monica
Farsang, Attila
Katinger, Dietmar
Moore, John P.
Scarlatti, Gabriella
Shattock, Robin J.
Sattentau, Quentin J.
Sanders, Rogier W.
机构：
[1] Department of Medical Microbiology, Amsterdam Institute for Infection and Immunity, Amsterdam UMC, University of Amsterdam, Amsterdam
[2] Control Laboratory of Veterinary Medicinal Products and Animal Facility, Directorate of Veterinary Medicinal Products, National Food Chain Safety Office, Budapest
[3] Polymun Scientific Immunbiologische Forschung GmbH, Klosterneuburg
[4] Viral Evolution and Transmission Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS Ospedale San Raffaele, Milan
[5] Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY
[6] Imperial College London, Department of Medicine, Division of Infectious Diseases, Section of Virology, Norfolk Place, London
[7] The Sir William Dunn School of Pathology, The University of Oxford, Oxford
来源：
NPJ VACCINES | 2021年 / 6卷 / 01期
基金：
美国国家卫生研究院; 欧盟地平线“2020”;
关键词：
D O I：
10.1038/s41541-021-00398-1
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The immunogenicity of HIV-1 envelope (Env) trimers is generally poor. We used the clinically relevant ConM SOSIP trimer to compare the ability of different adjuvants (squalene emulsion, ISCOMATRIX, GLA-LSQ, and MPLA liposomes) to support neutralizing antibody (NAb) responses in rabbits. The trimers were administered as free proteins or on nanoparticles. The rank order for the adjuvants was ISCOMATRIX > SE > GLA-LSQ ~ MPLA liposomes > no adjuvant. Stronger NAb responses were elicited when the ConM SOSIP trimers were presented on ferritin nanoparticles. We also found that the GLA-LSQ adjuvant induced an unexpectedly strong antibody response to the ferritin core of the nanoparticles. This “off-target” effect may have compromised its ability to induce the more desired antitrimer antibodies. In summary, both adjuvants and nanoparticle display can improve the magnitude of the antibody response to SOSIP trimers but the best combination of trimer presentation and adjuvant can only be identified experimentally. © 2021, The Author(s).
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页数：1
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