Prevalidation study of the Syrian hamster embryo (SHE) cell transformation assay at pH 7.0 for assessment of carcinogenic potential of chemicals

被引：9

作者：

Maire, Marie-Aline ^{[1
]}

Pant, Kamala ^{[2
]}

Poth, Albrecht ^{[3
]}

Schwind, Karl-Rainer ^{[4
]}

Rast, Claudine ^{[1
]}

Bruce, Shannon W. ^{[2
]}

Sly, Jamie E. ^{[2
]}

Kunz-Bohnenberger, Susanne ^{[3
]}

Kunkelmann, Thorsten ^{[3
]}

Engelhardt, Guenter ^{[4
]}

Schulz, Markus ^{[4
]}

Vasseur, Paule ^{[1
]}

机构：

[1] Univ Paul Verlaine Metz, CNRS, UMR 7146, Lab Interact Ecotoxicol Biodivers Ecosyst, F-57070 Metz, France

[2] BioReliance, Rockville, MD 20850 USA

[3] Harlan Cytotest Cell Res GmbH Harlan CCR, D-64380 Rossdorf, Germany

[4] BASF SE, Expt Toxicol & Ecol, D-67056 Ludwigshafen, Germany

来源：

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 2012年 / 744卷 / 01期

关键词：

Alternative method; pH; 7.0; Cell transformation assay; Syrian hamster embryo cells; Inter-laboratory comparison; MORPHOLOGICAL-TRANSFORMATION; CODED CHEMICALS; IN-VITRO; SUPERINDUCTION; HYDROCARBONS; EXPRESSION; APOPTOSIS; TESTS; ZNCL2;

D O I：

10.1016/j.mrgentox.2011.12.007

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The European Centre for the Validation of Alternative Methods (ECVAM) has organised an interlaboratory prevalidation study on the Syrian hamster embryo (SHE) cell transformation assay (CIA) at pH 7.0 for the detection of rodent carcinogens. The SHE CIA at pH 7.0 has been evaluated for its within-laboratory reproducibility, transferability and between-laboratory reproducibility. Four laboratories using the same basic protocol with minor modifications participated in this study and tested a series of six coded-chemicals: four rodent carcinogens (benzo(a)pyrene, 3-methylcholanthrene, 2,4-diaminotoluene and o-toluidine HCl) and two non-carcinogens (anthracene and phthalic anhydride). All the laboratories found the expected results with coded chemicals except for phthalic anhydride which resulted in a different call in only one laboratory. Based on the outcome of this study, it can be concluded that a standardised protocol is available that should be the basis for future use. This protocol and the assay system itself are transferable between laboratories and the SHE CIA at pH 7.0 is reproducible within- and between-laboratories. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：64 / 75

页数：12

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