In situ forming chitosan-based hydrogel as a lung sealant for biological lung volume reduction

被引：15

作者：

Songkroh, Titima ^{[1
,2
]}

Xie, Hongguo ^{[1
]}

Yu, Weiting ^{[1
]}

Lv, Guojun ^{[1
]}

Liu, Xiudong ^{[3
]}

Wang, Lin ^{[4
]}

Sun, Guangwei ^{[1
]}

Xu, Xiaoxi ^{[1
]}

Ma, Xiaojun ^{[1
]}

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China

[4] Dalian Univ, Affiliated Zhongshan Hosp, Dalian 116001, Peoples R China

来源：

SCIENCE BULLETIN | 2015年 / 60卷 / 02期

关键词：

Chitosan-based hydrogels; Biological lung volume reduction; Lung sealants; Emphysema; ADVANCED EMPHYSEMA; TISSUE; BIOMATERIALS; DELIVERY; THERAPY; GELS;

D O I：

10.1007/s11434-014-0548-3

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Biological lung volume reduction (BLVR) using lung sealant has received more attention recently as a new non-surgical approach to emphysema treatment. Many tissue sealants have been studied but only a few have been proposed for BLVR. In this work, we prepared in situ forming chitosan-based hydrogels (CSG) using covalent cross-linking of chitosan and genipin in the cooperation of ionic interaction between chitosan and sodium orthophosphate hydrate (Na3PO4 center dot 12H(2)O) and characterized by Fourier transform infrared spectroscopy, scanning electron microscopy and rheological methods. CSG showed short gelation time (8 min), high swelling ratio (> 100 %) and non-toxicity (3T3 mouse fibroblast cell viability > 80 %) under physiological conditions. The application of lung sealant for BLVR was tested in a Chinese dog and evaluated by chest computed tomography. After 3 weeks of the installation of CSG in bronchopulmonary segment, the gel formation was detected at a localized region of bronchi and the local atelectasis occurred. Our findings indicate that this chitosan-based hydrogel is a promising new candidate for use as a lung sealant for BLVR.

引用

页码：235 / 240

页数：6